PROTEIN INTERACTIONS IN YEAST VACUOLE FUSION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们对参与酵母液泡膜融合的几种蛋白质的作用很感兴趣，包括Rab GTP酶(Ypt7p)、其效应复合体(HOPS)和空泡陷阱蛋白(Vam3p、Vam7p、Vti1p、Nyv1p)。我们正在制作双杂交诱饵，其中包括通过生物信息学分析确定的这些蛋白质的假定亚域。我们的目标是生成已知对接和融合蛋白质的蛋白质相互作用图，并确定新的相互作用因子。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in the roles of several proteins involved in yeast vacuole membrane fusion, including a Rab GTPase (Ypt7p), its effector complex (HOPS), and vacuolar SNARE proteins (Vam3p, Vam7p, Vti1p, Nyv1p). We are generating 2-hybrid baits that include putative subdomains of these proteins identified through bioinformatic analyses. Our goal is to generate a protein interaction map of known docking and fusion proteins, and to identify new interactors.