FLOW CYTOMETRY CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。流式细胞术核心设施是一个新组织的核心，现在功能齐全，为NCV成员和相关人员提供流式细胞术的技术和功能支持。由核心控制的仪器有两个位置，Beadle中心的BL3设施和兽医基础科学大楼的流式细胞术实验室。姐妹核心实验室位于内布拉斯加大学医学中心校园的达勒姆研究中心。该设施现在包括一台FACSCalibur，四色台式分析仪器，一台FACScan，三色台式分析仪器和一台FACS Vantage 6彩色细胞分选仪器，全部来自Becton Dickinson Biosciences。这些仪器为NCV研究人员提供了广泛的应用，包括免疫荧光、头捕获测定、DNA谱和细胞分选。数据被收集并以列表模式文件的形式存储在核心的现场。所有的数据被分析并返回给研究者供他们批准。此外，关于新协议开发的深入讨论是一个正在进行的过程。在这一年中，进行了协商，以提交新的赠款和出版物。目前有两名技术人员，每个地点一名，提供支持和获取数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Flow Cytometry Core Facility is a newly organized core that is now fully functioning and provides NCV members and associates with technical and functional support for flow cytometry. There are two locations for the instrumentation controlled by the core, the BL3 facility in the Beadle Center and the flow cytometry lab in the Veterinary Basic Science building. A sister core laboratory is located in the Durham Center for Research on the University of Nebraska Medical Center Campus. The facility now consists of a FACSCalibur, four color bench top analysis instrument, a FACScan, three color bench top analysis instrument and a FACS Vantage 6 color cell sorting instrument, all from Becton Dickinson Biosciences. These instruments provide a wide range of applications for NCV investigators, including immunofluorescence, bead capture assays, DNA profiles and cell sorting. The data are collected and stored as list mode files on site in the core. All data is analyzed and returned to the investigators for their approval. In addition, in depth discussions regarding new protocol development are an ongoing process. During this year, consultations have taken place leading to new grant submissions and publication submissions. Currently there are two echnicians, one at each location to provide support and acquire data.