COBRE: RIH: CORE B: PROTEOMICS: PROTEIN BIOINFORMATICS, HU GENOME, DNA

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。蛋白质组学核心为这个COBRE癌症中心、位于罗德岛的其他COBRE中心和更大的学术界的成员的研究工作提供了最先进的仪器和支持。我们的收费服务包括质谱分析，如LC/MS/MS和SELDI-TOF质谱分析，电泳分析，包括SDS-PAGE，等电聚焦，二维电泳，免疫印迹和使用VersaDoc成像系统的图像分析，以及蛋白质和肽层析和肽合成。我们还提供蛋白质纯化和表征以及小规模下游加工方面的专业知识。我们的设施位于国际扶轮医院的Coro中心。今年，共有23个研究小组使用我们的服务和设施。目前有7位研究人员在我们的实验室使用仪器，主要是质谱仪，Ciphergen SELDI-TOF色谱系统进行样品的分离，鉴定和表征。我们的两个质谱仪，来自ABI的QStar XL LC/MS/MS系统和来自Ciphergen的SELDI-TOF都是常规使用的。SELDI-TOF系统也可用于非蛋白质组学核心研究人员。我们最近还培训了四名来自COBRE其他实验室的年轻研究人员使用该仪器。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Proteomics Core provides state-of-the art instrumentation and support for the research efforts of the members of this COBRE Cancer Center, other COBRE Centers located in Rhode Island and greater academic community. Our fee-based services include mass spectrometric analyses such as LC/MS/MS and SELDI-TOF mass spectrometry, electrophoretic analyses, including SDS-PAGE, isoelectric focusing, 2-D electrophoresis, immunoblot, and image analysis by use of the VersaDoc Imaging System, and protein and peptide chromatography and peptide synthesis. We also offer expertise in protein purification and characterization, and small-scale down stream processing. Our facility is located in the Coro Center at RI Hospital. In this year, a total of 23 research groups have made use of our services and facilities. Seven investigators currently use instruments in our laboratory, mainly the mass spectrometer, Ciphergen SELDI-TOF chromatographic systems for isolation, identification and characterization of their samples. Both of our mass spectrometers, a QStar XL LC/MS/MS system from ABI and SELDI-TOF from Ciphergen are in routine use. The SELDI-TOF system is also available for non-proteomics core investigators. We have also newly trained four young investigators from other COBRE laboratories to use this instrument.