KY COBRE: TISSUE CULTURE & PROTEIN PRODUCTION CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。组织培养和蛋白质生产核心旨在促进COBRE研究人员的细胞培养和重组蛋白质生产，这是他们拟议项目的关键组成部分。它将提供维护良好的设备和仪器，这些设备和仪器通常不会在个别实验室，特别是初级研究人员的实验室中找到。办法是提供培训和优先接触调查小组成员，而不是以服务方式维持完成任务所需的人员。这样，成本就会降到最低。Core将为哺乳动物细胞培养提供组织培养罩和培养箱。此外，发酵罐、生物反应器、细胞裂解设备和色谱仪器将可用于生产重组蛋白。表征重组蛋白的仪器也将在核心中提供。一名工作人员将维护核心设备，为新用户提供培训，并监督调度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Tissue Culture and Protein Production Core is designed to facilitate cell culture and recombinant protein production by the COBRE investigators, key components of their proposed projects. It will make available well-maintained equipment and instrumentation that would not normally be found within individual laboratories, particularly those of junior investigators. The approach is to provide training and priority access to members of the investigators'' teams rather than maintaining the personnel required to accomplish the tasks on a service basis. In this way, costs will be kept to a minimum. The Core will provide tissue culture hoods and incubators for mammalian cell culture. In addition, fermenters, bioreactors, cell cracking equipment, and chromatography instrumentation will be available for production of recombinant proteins. Instrumentation to characterize recombinant proteins will also be available in the Core. A single staff member will maintain the Core equipment, provide training to new users, and oversee scheduling.