COBRE: LOUISVILLE RES FOUND INC: P1: GLYCOLYSIS AND NEOPLASTIC GROWTH

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。在过去的12个月里，我们研究了PFKFB 3的代谢和肿瘤的要求ras转化使用siRNA和杂合子基因组缺失技术的组合。我们发现，PFKFB 3的表达是有选择性地控制糖酵解通量与ras转化细胞所必需的，并提出PFKFB 3的药理学抑制可能会影响肿瘤细胞的生存和生长（见本报告中的子项目进展）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the past 12 months, we have examined the metabolic and neoplastic requirements of PFKFB3 for ras transformation using a combination of siRNA and heterozygote genomic deletion technologies. We find that PFKFB3 expression is selectively necessary for the control of glycolytic flux in cells transformed with ras and propose that pharmacologic inhibition of PFKFB3 may affect the survival and growth of neoplastic cells (see Subproject Progress in this report).