COBRE: LOUISVILLE RES FOUND INC: CORE B: MICROARRAY FACILITY

COBRE：LOUISVILLE RES FOUND INC：核心 B：微阵列设施

• 批准号: 8360664
• 负责人: Wolfgang Zacharias
• 金额: $ 11.6万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360664
• 关键词: Applications Grants; Bioinformatics; Biomedical Research; Cancer Center; Centers of Research Excellence; Characteristics; Computer software; Core Facility; Data Analyses; Data Collection; Development; Educational workshop; Experimental Designs; Funding; Generations; Genome; Grant; Human; Human Resources; Logistics; Microarray Analysis; Molecular Target; Mus; National Center for Research Resources; Pathologic; Preparation; Principal Investigator; Process; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Sampling; Source; Specimen; System; Time; Tissue Microarray; Tissues; Training; United States National Institutes of Health; biobank; cost; density; instrument; member

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Microarray Facility is located in the Baxter II Biomedical Research Building, one block from the Brown Cancer Center. This facility contains both Affymetrix and Agilent microarray instrument systems which are available to all MT-COBRE members. The Facility is under the direction of Dr. Wolfgang Zacharias and has been fully operational for the past eight years since Spring 2001. It includes a full-time Facility Manager, Sabine Waigel, and two research technologists, Vennila Arumugam and Xiaohong Li. They provide administrative support, project logistics and management, technical assistance in platform-specific sample processing and hybridization, as well as data collection, analysis and storage. Dr. Zacharias is available for assistance with experimental design, data analysis, and collaborative or consultant support for grant applications. The Microarray Facility has close interactions with other Core Facilities and Bioinformatics personnel. Also, the close relationship with the Tissue Biorepository in the J.G. Brown Cancer Center allows the preparation of samples with known pathologic characteristics and appropriate control specimens. Plans are underway for the development of a tissue array component of this facility as well. The high-resolution Affymetrix Scanner 3000 upgrade allows all COBRE investigators to use the newest generation of high-density whole genome v2.0 arrays and SNP 6.0 arrays for human or mouse. The Facility continues to organize and hold software/data analysis workshops on campus to train microarray technology users.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 微阵列设施位于巴克斯特II生物医学研究大楼，距离布朗癌症中心一个街区。该设施包含Affyoung和Agilent微阵列仪器系统，可供所有MT-COBRE成员使用。该基金由沃尔夫冈·扎卡里亚斯博士领导，自2001年春季以来已全面运作八年。该团队包括一名全职设施经理Sabine Waigel以及两名研究技术专家Vennila Arumugam和Xiaohong Li。他们提供行政支持、项目后勤和管理、特定平台样品处理和杂交方面的技术援助，以及数据收集、分析和存储。Zacharias博士可提供实验设计，数据分析以及资助申请的协作或顾问支持。 微阵列设施与其他核心设施和生物信息学人员密切互动。此外，与J.G.组织生物储存库的密切关系。布朗癌症中心允许制备具有已知病理特征的样本和适当的对照标本。目前正在计划开发该设施的组织阵列组件。高分辨率的Affyscanner 3000升级版允许所有COBRE研究人员使用最新一代的高密度全基因组v2.0阵列和SNP 6.0阵列用于人类或小鼠。该基金继续在校园内组织和举办软件/数据分析讲习班，培训微阵列技术用户。