COBRE: LOUISVILLE RES FOUND INC: CORE B: MICROARRAY FACILITY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 微阵列设施位于巴克斯特II生物医学研究大楼，距离布朗癌症中心一个街区。该设施包含Affyoung和Agilent微阵列仪器系统，可供所有MT-COBRE成员使用。该基金由沃尔夫冈·扎卡里亚斯博士领导，自2001年春季以来已全面运作八年。它包括一名全职设施经理Sabine Waigel和两名研究技术专家Vennila Arumugam和Xiaohong Li。他们提供行政支持、项目后勤和管理、特定平台样品处理和杂交方面的技术援助，以及数据收集、分析和存储。Zacharias博士可提供实验设计，数据分析以及资助申请的协作或顾问支持。 微阵列设施与其他核心设施和生物信息学人员密切互动。此外，与J.G.组织生物储存库的密切关系。布朗癌症中心允许制备具有已知病理特征的样本和适当的对照标本。目前正在计划开发该设施的组织阵列组件。高分辨率的Affyscanner 3000升级版允许所有COBRE研究人员使用最新一代的高密度全基因组v2.0阵列和SNP 6.0阵列用于人类或小鼠。该基金继续在校园内组织和举办软件/数据分析讲习班，培训微阵列技术用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray Facility is located in the Baxter II Biomedical Research Building, one block from the Brown Cancer Center. This facility contains both Affymetrix and Agilent microarray instrument systems which are available to all MT-COBRE members. The Facility is under the direction of Dr. Wolfgang Zacharias and has been fully operational for the past eight years since Spring 2001. It includes a full-time Facility Manager, Sabine Waigel, and two research technologists, Vennila Arumugam and Xiaohong Li. They provide administrative support, project logistics and management, technical assistance in platform-specific sample processing and hybridization, as well as data collection, analysis and storage. Dr. Zacharias is available for assistance with experimental design, data analysis, and collaborative or consultant support for grant applications. The Microarray Facility has close interactions with other Core Facilities and Bioinformatics personnel. Also, the close relationship with the Tissue Biorepository in the J.G. Brown Cancer Center allows the preparation of samples with known pathologic characteristics and appropriate control specimens. Plans are underway for the development of a tissue array component of this facility as well. The high-resolution Affymetrix Scanner 3000 upgrade allows all COBRE investigators to use the newest generation of high-density whole genome v2.0 arrays and SNP 6.0 arrays for human or mouse. The Facility continues to organize and hold software/data analysis workshops on campus to train microarray technology users.