COBRE: LOUISVILLE RES FOUND INC: CORE B: MICROARRAY FACILITY

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 微阵列设施位于 Baxter II 生物医学研究大楼，距离布朗癌症中心仅一个街区。该设施包含 Affymetrix 和 Agilent 微阵列仪器系统，可供所有 MT-COBRE 成员使用。该设施由 Wolfgang Zacharias 博士领导，自 2001 年春季以来已全面运营八年。该设施包括一名全职设施经理 Sabine Waigel 和两名研究技术专家 Vennila Arumugam 和 Xiaohong Li。他们提供行政支持、项目物流和管理、特定平台样品处理和杂交方面的技术援助，以及数据收集、分析和存储。 Zacharias 博士可在实验设计、数据分析以及拨款申请的协作或顾问支持方面提供帮助。 微阵列设施与其他核心设施和生物信息学人员有着密切的互动。此外，与 J.G. 组织生物储存库的密切关系。布朗癌症中心允许制备具有已知病理特征的样本和适当的对照样本。该设施的组织阵列组件的开发计划也正在进行中。高分辨率 Affymetrix Scanner 3000 升级允许所有 COBRE 研究人员使用最新一代的人类或小鼠高密度全基因组 v2.0 阵列和 SNP 6.0 阵列。该设施继续在校园内组织和举办软件/数据分析研讨会，以培训微阵列技术用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray Facility is located in the Baxter II Biomedical Research Building, one block from the Brown Cancer Center. This facility contains both Affymetrix and Agilent microarray instrument systems which are available to all MT-COBRE members. The Facility is under the direction of Dr. Wolfgang Zacharias and has been fully operational for the past eight years since Spring 2001. It includes a full-time Facility Manager, Sabine Waigel, and two research technologists, Vennila Arumugam and Xiaohong Li. They provide administrative support, project logistics and management, technical assistance in platform-specific sample processing and hybridization, as well as data collection, analysis and storage. Dr. Zacharias is available for assistance with experimental design, data analysis, and collaborative or consultant support for grant applications. The Microarray Facility has close interactions with other Core Facilities and Bioinformatics personnel. Also, the close relationship with the Tissue Biorepository in the J.G. Brown Cancer Center allows the preparation of samples with known pathologic characteristics and appropriate control specimens. Plans are underway for the development of a tissue array component of this facility as well. The high-resolution Affymetrix Scanner 3000 upgrade allows all COBRE investigators to use the newest generation of high-density whole genome v2.0 arrays and SNP 6.0 arrays for human or mouse. The Facility continues to organize and hold software/data analysis workshops on campus to train microarray technology users.