CORE C: HISTOPATHOLOGY CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。该中心为Cobre和其他MMCRI研究人员提供组织学服务。该芯体对冷冻、石蜡或塑料包埋的样品进行组织固定、包埋和切片。核心进行组织学染色，包括苏木精-伊红，三色，von Kossa，地衣，van Gieson(和其他需要的)。用各种抗体进行免疫组织化学染色也是核心服务的一部分。核心由一位在该领域有超过25年经验的全职历史学家操作(凯瑟琳·卡里尔)。CORE主管(Volkhard Lindner)监督CORE的运作，并协助解释和评估组织标本。Core使用以下设备：用于冰冻切片的Microm HM505E；用于石蜡和塑料切片的Microm HM355S和Microm HM325；用于石蜡渗透、幻灯片加热器和水浴的Lab-Line 3618型真空烤箱和VWR 1410型真空烤箱。对于集中式组织学手术，一台自动组织处理机(徕卡TP1020)于2004年通过科布尔奖提供的资金投入使用。2005年，一台自动玻片着色机(徕卡AUTOSTINER XL)和盖子滑移站(Hyperlean)上线。凯思琳·凯利执行所有日常操作，包括组织处理(徕卡TP1020)和特殊染色；免疫组织化学；申请表；订购和维护记录；选择方案开发；以及共焦显微镜。此外，在线还有HM505E低温恒温器、HM355S和HM325显微切割机、组织中心包埋站和用于石蜡渗透的烤箱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides histology services to COBRE and other MMCRI investigators. The Core performs tissue fixation, embedding, and sectioning of frozen, paraffin or plastic embedded specimens. The Core performs histological stains including Hematoxylin-Eosin, Trichrome, von Kossa, Orcein, van Gieson (and others as needed). Immunohistochemical staining with a variety of antibodies is also part of Core services. The Core is operated by a full time histologist with more than 25 years of experience in the field (Kathleen Carrier). The Core director (Volkhard Lindner) oversees Core operations and assists in the interpretation and evaluation of histology specimens. The Core uses the following equipment: Microm HM505E Cryostat for frozen sections, A Microm HM355S and Microm HM325 microtome for paraffin and plastic sections, A Fisher HistoCenter embedding station, Lab-Line Vacuum oven Model 3618 and VWR vacuum oven model 1410 for paraffin infiltration, Slide Warmers and water baths. For to the centralized histology operation an automated tissue processor (Leica TP1020) was brought on line in 2004 through funds provided by this COBRE award. An automated slide stainer (Leica Autostainer XL) and cover-slipping station (Hyperclean) were brought online in 2005. Kathleen Carrier performs all day-to-day operations including tissue processing (Leica TP1020) & special stains; immunohistochemistry; request forms; ordering and maintenance records; select protocol development; and confocal microscopy. Also, on-line are a HM505E Cryostat, HM355S and HM325 microtomes, HistoCenter embedding station, and ovens for paraffin infiltration.