DARTMOUTH COL COBRE: PROTEOMICS FACILITY CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。蛋白质组学是我们COBRE项目5个初始研究项目中的4个的主要组成部分，也是该项目高级导师的研究组成部分。我们的目标是开发一个具有完整系列蛋白质组学相关服务的蛋白质组学设施核心。在短短的一年里，我们开始开发和扩展蛋白质组学核心的仪器和方法，收购了Biacore X SPR生物传感器来进行表面等离子体共振研究（SPR）。我们还开始了利用COBRE奖的A&R资金对核心设施进行翻新的规划过程。2002年，我们继续为我们的研究人员扩展和开发蛋白质组学相关的服务和方法，包括用于复杂蛋白质样品的2D凝胶电泳和PF2D液体分离技术，以及对ESI-MS注射系统的改进。我们还成功招募了一名生物信息学教授（J. Moore）作为COBRE项目的一部分，他将协助分析蛋白质组学数据。在核心的支持和帮助下，项目2和项目3已经开始在研究中使用蛋白质组学工具。我们现在正在与项目1和项目4一起讨论他们的蛋白质组学相关研究，并为他们的使用开发合适的方法和工具。我们在2002年将最终的改造计划提交给NIH COBRE工作人员进行最终批准，并开始获得当地A&R许可以及最终设计合同的过程。我们现已开始翻修工程，工程的三个阶段之一已接近完成，另外两个阶段已开始进行。我们预计这些阶段将在未来4-6个月内完成。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proteomics is a major component of four of the five initial research projects of our COBRE program, as well as a component of the research of the senior mentors of this program. Our goal is to develop a Proteomics Facility Core with a complete series of proteomics-related services. In the shortened year 01 we began to develop and expand the Proteomics Core's instrumentation and methodologies with the acquisition of a Biacore X SPR biosensor to perform surface plasmon resonance studies (SPR). We also began the planning process for renovation of the core facilities using the A&R funds from this COBRE award. In year 02 we have continued to expand and develop proteomics related services and methodologies for our investigators, including 2D gel electrophoretic and PF2D liquid separation techniques for complex protein samples and improvements to the ESI-MS injection system. We also successfully recruited a bioinformatics faculty (J. Moore) as part of the COBRE program who will assist with analysis of proteomics data. Projects 2 and 3 have begun using proteomics tools in their research with the core's support and assistance. We are now working with Projects 1 and 4 to discuss their proteomics related research and develop appropriate methods and tools for their use. We submitted the finalized renovation plans to NIH COBRE staff in year 02 for final approval, and began the process of obtaining local A&R permitting as well as contracting for final design. We have now begun the renovation process, with completion nearing for one of the three phases of the project and initiation of the other two phases. We anticipate completion of these phases over the next 4-6 months.