COBRE: LSC HSC: HISTOLOGY CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。组织学核心总监<$Jack Strong，医学博士 组织学核心在Jack P. Strong，M.D.的指导下，在组织学和心血管病理学各方面具有丰富经验的研究者。该组织学核心负责组织处理和染色，动物模型以及细胞培养的病理学分析，以及组织和细胞样本的原位杂交和免疫细胞化学。所有初级研究人员都使用该核心进行细胞和组织分析。从固定灌注动物或速冻器官中制备组织切片。我们还通过访问LSUHSC形态核心设施中的其他资源来扩展此资源。该核心中的组织处理设备包括Hypercenter XP处理器; Histocentre石蜡包埋站; Finesse石蜡切片机;配备用于整个小鼠切片的碳化钨刀的Leica CM 3050 S低温恒温器和EMS振荡组织切片机。 组织学核心提供了以下服务：1。心脏、肾脏和血管组织处理;组织的苏木精、伊红和亚甲蓝染色。2.大鼠对照和受损动脉组织（平滑肌肌动蛋白和Erk-1）的免疫组织化学染色。3.利用平滑肌肌动蛋白4评估损伤与对照大鼠动脉组织以及对血管内膜平滑肌细胞的影响。损伤动脉组织与对照动脉组织中T细胞和巨噬细胞的基于免疫组织化学的评估5. TUNEL染色分析心肌细胞损伤和死亡。 通过Strong和Huber博士的努力，核心实验室提供了所有适当的技术和经过专门培训的人员，以在显微镜和分子水平上评价来自不同基质上的各种固定的人和小鼠动脉标本和细胞培养物的这些标本。这一核心设施的使用将在未来一年继续增长。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Histology Core Director ¿ Jack Strong, M.D. The Histology Core is under the direction of Jack P. Strong, M.D., an investigator who has extensive experience in all aspects of histology and cardiovascular pathologies. This Histology Core is responsible for tissue processing and staining, pathology analysis in animal models as well as cell cultures, and in situ hybridization and immunocytochemistry in tissue and cell samples. This core is used by all junior investigators for cell and tissue analysis. Tissue sections are generated from either fixative-perfused animals or from snap-frozen organs. We have also expanded this resource by accessing additional resources in the LSUHSC morphology core facility. Tissue processing equipment in this core includes a Hypercenter XP processor; Histocentre paraffin embedding station; Finesse paraffin microtome; a Leica CM3050 S cryostat equipped with a tungsten carbide knife for whole mouse sectioning and an EMS oscillating tissue slicer. The histology core rendered the following services: 1. Cardiac, renal and vascular tissue processing; Hematoxylin and eosin and methylene blue staining of tissues . 2. Immunohistochemical staining of rat control and damaged arterial tissues (smooth muscle actin and Erk-1). 3. Evaluation of injury-versus-control rat arterial tissues and the effects exerted upon smooth muscle cells in vascular intima using smooth muscle actin 4. Immunohistochemical-based assessments of T-cells and macrophages in injury-versus-control arterial tissues 5. Analysis of cell damage and death in myocardium by TUNEL stains. The core lab, via the efforts of Drs. Strong and Huber, provides all of the appropriate technology and specially trained personnel to evaluate these specimens on a microscopic and molecular level from various fixed human and murine arterial specimens and cell cultures on different matrices. The use of this core facility will continue to grow in the upcoming year.