Establishment of a SPF Rhesus Macaque Colony

描述（由申请人提供）：该项目的目标是通过维持一个具有B型疱疹病毒、SIV、SRV和STLV-1的SPF的印度猕猴生产群体，提高国家生产无特定病原体纯种猕猴（SPF）用于艾滋病相关研究的能力，并通过提供具有MHC基因型特征的动物来满足近期和长期的特定研究需求。具体目的如下：1。通过详细的评估和去除阳性动物的计划，为nih资助的研究人员提供以下数量的动物：06年60只，07年60只，08年70只，09年70只，10年70只。该群体将扩大到大约430只动物的稳定种群，其中约140只雌性繁殖动物，20只雄性繁殖动物，275只幼崽和幼崽，每年可以持续收获约70只动物用于研究；2. 通过向研究人员提供与以下Mamu等位基因相关的动物，使动物的研究价值最大化：Mamu-A*01， -A*02, -A*11, -B*01, -6*03， -B*04和b *17；3. 通过建立、管理和利用系谱数据库对菌落进行遗传管理，最大限度地提高菌落生产的长期效率。前5年通过NIH/NCRR的其他资助开发的20个信息丰富的微卫星多态性小组，以及建立该数据库的人口统计记录，将用于建立和验证系谱信息，管理育种以避免近亲繁殖和保持遗传变异，同时通过利用群体中的死亡率，发病率和生育力数据，将重点放在最大化生产和收获计划的主要目标上。

DESCRIPTION (provided by applicant): The goals of this project are to increase the nation's capacity to produce pedigreed specific pathogen free (SPF) Macaca mulatta for AIDS-related research by maintaining a production colony of Indian-origin rhesus monkeys that are SPF for herpes B virus, SIV, SRV, and STLV-1, and to fulfill specific research needs in the immediate and long-term future by providing animals characterized relative to MHC genotype. The specific aims are as follows: 1. Through a detailed program of assessment and removal of positive animals, to provide the following numbers of animals to NIH-funded investigators: 60 in Year 06, 60 in Year 07, 70 in Year 08, 70 in Year 09 and 70 in Year 10. The colony will expand to a steady-state population of around 430 animals, with approximately 140 breeder females, 20 breeder males, and 275 infants and juveniles, enabling a continuing annual harvest of approximately 70 animals for research; 2. To maximize the value of the animals for research purposes by offering to investigators animals that are characterized relative to the following Mamu alleles: Mamu-A*01, -A*02, -A*11, -B*01, -6*03, -B*04, and-B*17.; 3. To maximize the long term efficiency of colony production by a) establishing, managing, and utilizing a pedigree database for genetic management of the colony. The panel of 20 highly informative microsatellite polymorphisms developed in the first 5 years through other funding from NIH/NCRR, along with demographic records to establish this database, will be used to establish and verify pedigree information, manage breeding to avoid inbreeding and maintain genetic variability, while focusing on the primary goal of maximizing production and harvest schedules by utilizing data on mortality, morbidity, and fertility in the colony.