MOLECULAR NMR

描述(申请人提供)：分子核磁共振共享设施是由综合癌症中心建立的最初的共享设施之一，为该中心的教职员工提供高质量的样本分析。该设施的主要目标是提供“最先进的”仪器，供中心成员在其个人研究计划和与中心核心计划相关的研究中使用。该设施为教职员工提供培训和教育活动，以确保适当利用现有资源。此外，该设施还提供实验设计、样品制备和技术支持方面的专业知识。在之前的资助期间，该设施80%的使用是为癌症中心成员提供的。这些利用设施资源进行的研究包括：抗癌药物、多肽和抗体的各种连接物的设计和合成；药物-DNA复合体的结构表征；抗癌和抗病毒蛋白的研究，如干扰素-tau；HIV gp 160介导的T细胞凋亡的研究；用于癌症治疗的多西红素-多肽结合物的设计和评价；支架蛋白的功能结构域的研究；以及抗癌剂核苷类似物的设计、合成和分析。该设施寻求通过升级现有仪器和增加服务来扩大其为所有现有项目提供高质量核磁共振分析的能力。

DESCRIPTION (provided by applicant): The Molecular NMR Shared Facility was one of the original shared facilities established by the Comprehensive Cancer Center to provide high quality sample analysis to the Center's faculty membership. The primary objectives of the facility are to provide "state-of-art" instrumentation for use by the Center members in their individual research programs and research related to the Center's Core Programs. The facility provides training and educational activities for faculty and staff to insure proper utilization of the resources available. In addition, the facility provides expertise in experimental design, sample preparation and technical support. During the previous funding period, 80% of the Facility usage was for Cancer Center members. These investigations conducted with facility resources involved work on the design and synthesis of various linkers for anticancer drugs, peptides and antibodies; structural characterization of drug-DNA complexes; studies on anticancer and antiviral proteins, such as interferon-tau; investigations on the HIV gp 160-mediated apoptosis of T cells; design and evaluation of Doxirubin-peptide conjugates for cancer treatments; Studies on the functional domains of scaffolding proteins; and the design, synthesis and analysis of nucleoside analogs of anticancer agents. The facility seeks to expand its ability to provide quality NMR analysis for all existing projects through upgrading existing instrumentation and increased services.