MASS SPECTROSCOPY/PROTEOMICS

描述（由申请人提供）：质谱/蛋白质组学共享设施是 组织提供各种分析和技术服务，使用质谱UAB综合癌症中心的研究人员。 该共享设施目前有五个质量 光谱仪-两台MALD 1-TOF仪器，两台三重四极杆仪器和一台混合 四极正交TOF仪器。 2004年夏季将安装三台新仪器（一台新的三重四极仪和两台傅立叶变换离子回旋共振仪-由国家癌症研究中心赠款支助）。 除了小分子（治疗和外源性物质）和肽的定性和定量分析外，质谱组还提供蛋白质化学和蛋白质表达领域的各种分析。 这些包括H-D交换以探测溶液中的蛋白质结构以及蛋白质-蛋白质相互作用。 最近还增加了程序，使研究人员能够追求蛋白质组学的应用。 共享设施的蛋白质组学部分将为研究者提供咨询意见，如如何准备分析样品，并在大多数情况下进行分析。 这包括从细胞或组织样品中回收蛋白质组，使用1D-SDS-PAGE或2D-IEF/SDS-PAGE解析蛋白质，对凝胶进行染色和成像，以及进行复杂的图像分析以检测可观察到的蛋白质组中发生的变化。 蛋白质组 通过生物统计学/生物信息学共享设施的统计分析对这些进行验证。通过机器人去除蛋白质斑点，并在机器人处理后通过MALDI-TOF质谱法生成蛋白酶诱导的肽谱分析。 用MASCOT搜索引擎对肽质量指纹进行统计分析，以鉴定蛋白质。 通过肽的nano-LC串联质谱法进行蛋白质同一性验证。 该方法还允许检测翻译后修饰。

DESCRIPTION (provided by applicant): The Mass Spectrometry/Proteomics Shared Facility is organized to provide a variety of analytical and technical services using mass spectrometry to UAB Comprehensive Cancer Center investigators. The Shared Facility currently has five mass spectrometers - two MALD1-TOF instruments, two triple quadrupole instruments, and one hybrid quadrupole orthogonal TOF instrument. Three new instruments (a new triple quadrupole instrument and two fourier transform-ion cyclotron resonance instruments - supported by NCRR grants) will be installed during the summer of 2004. Besides qualitative and quantitative analysis of small molecules (therapeutic and xenobiotics) and peptides, the mass spectrometry group provides a variety of analyses in the area of protein chemistry and protein expression. These include H-D exchange to probe protein structure in solution as well as protein-protein interactions. Procedures have also recently been added to allow investigators to pursue proteomics applications. The proteomics section of the Shared Facility will provide investigators with consultant advice as how to prepare samples for analysis, and in most cases to carry out the analyses. This includes recovery of the proteome from the cell or tissue samples, resolving proteins using 1D-SDS-PAGE or 2D-IEF/SDS-PAGE, staining and imaging the gels, and carrying out sophisticated image analysis to detect changes that have occurred in the observable proteome. These are verified by statistical analysis by the Biostatistics/Bioinformatics Shared Facility. Protein spots are removed robotically and following robotic processing to generate a protease-induced peptide profile analysis by MALDI-TOF mass spectrometry. The peptide mass fingerprint is subjected to statistical analysis with the MASCOT search engine to putatively identify the protein. Verification of protein identity is performed by nano-LC tandem mass spectrometry of the peptides. This method also allows for the detection of post-translational modifications.