Arrays for Protease Activity Profiling

描述（由申请人提供）：需要新技术来实现蛋白酶活性的高分辨率、多路分析。这一领域的进展有可能使发现细胞功能和调节的基本新见解成为可能。我们建议开发一种新的技术，利用蛋白酶底物肽微阵列对蛋白酶活性进行敏感和特异性的分析。我们将开发针对高通量蛋白酶活性分析优化的阵列制备、肽标记和检测方法。

DESCRIPTION (provided by applicant): There is a need for new technologies to enable high-resolution, multiplexed analysis of protease activity. Advances in this area have the potential to enable the discovery of fundamental new insights into cellular function and regulation. We propose to develop a novel technology for sensitive and specific analysis of protease activity using protease substrate peptide microarrays. We will develop methods for array preparation, peptide labeling and detection that are optimized for high-throughput protease activity analysis. PUBLIC HEALTH RELEVANCE: The system we are developing for protease activity analysis will help to enable the deciphering of cellular regulation of protein degradation and the relationship to phenotype. It will help to identify disorders in cellular regulation that lead to disease, and therefore may result in medical advances in the diagnosis and treatment of disease.