OPERATION AND MAINTENANCE OF A PROTEIN DATABASE SEARCH SERVER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 一个带有吉祥物软件的服务器被用于分析我们与Unanue和Yarashevski小组合作的免疫学项目中的肽和蛋白质序列。我们用LC-MS/MS分析多肽混合物或消化的蛋白质，并提交光谱进行分析，以确定抗原肽的序列和蛋白质来源，并用于肌肉蛋白质组计划。了解抗原的性质有助于对抗糖尿病等自身免疫性疾病。 该服务器正被用于许多其他项目，包括HDX和蛋白质足印等核心项目，肌肉等待和阿尔茨海默病的合作项目，以及蛋白质识别服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A server with MASCOT software is used to analyze peptide and protein sequences for our collaborative immunology project with the Unanue and Yarashevski groups. We analyze peptide mixtures or digested proteins by LC-MS/MS and submit the spectra for analysis to determine the sequences and protein origin of antigenic peptides and for the muscle proteom project. Knowledge of the nature of antigens can help fight autoimmune diseases like diabetes. The server is being used for many other projects that include core projects like HDX and foot printing of proteins, collaborative projects on muscle waisiting and Alzhiemer disease and service for protein identifications.