CHAR OF CARDIOLIPIN FROM ESCHERICHIA COLI BY ELECTROSPRAY IONIZATION WITH

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们报告了多级离子阱（IT）质谱电喷雾电离（ESI）的心磷脂（CL）的[M -2 H + Na]-离子的结构表征方法，1，3-双磷脂酰-sn-甘油，由四个脂肪酰基链和三个甘油骨架指定，即，A，B和中央甘油，分别。CAD后，CL的[M -2 H + Na]-离子的MS 2光谱包含两个主要碎片离子，这两个碎片离子是由涉及A或B甘油的相应二酰基甘油部分的差异损失引起的。两个磷脂酰部分连接到中心甘油的1 '或3'位的暂定归属是基于以下观察结果，即甘油B（或A）的二酰基甘油部分丢失产生的离子比甘油A（或B）的离子更丰富。这两种离子的结构，包括脂肪酰基取代基的身份和脂肪酰基取代基在甘油主链（甘油A和B）上的位置，通过它们的MS 3光谱来确定，MS 3光谱包括几组为其测定提供信息的主要离子。这种方法允许CL在从大肠杆菌分离的混合物中的结构，包括由各种异构体组成的物种，被详细揭示。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We report multiple-stage ion-trap (IT) mass spectrometric methods with electrospray ionization (ESI) for structural characterization of the [M - 2H + Na]- ion of cardiolipin (CL), a 1,3-bisphosphatidyl-sn-glycerol that consists of four fatty acyl chains and three glycerol backbones designated, namely, as A, B and central glycerol, respectively. Following CAD, the MS2-spectrum of the [M - 2H + Na]- ions of CL contains two prominent fragment ions resulting from the differential losses of the respective diacylglycerol moieties involving A or B glycerol. The tentative assignment of the two phosphatidyl moieties attached to the 1'- or 3'-position of the central glycerol is based on the observation that the ions arising from loss of the diacylglycerol moiety involving glycerol B (or A) is more abundant than that involving glycerol A (or B). The structures of the two ions, including the identities of the fatty acyl substituents and the position of fatty acyl substituents on the glycerol backbones (glycerol A and B) are determined by their MS3-spectra that comprise several sets of prominent ions informative for their determination. This method permits the structures of CL in a mixture isolated from Escherichia Coli, including species that consist of various isomers, to be unveiled in details.