GENERATING A PROTEIN INTERACTION MAP OF A VIRAL KILLER TOXIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 微生物毒素是许多严重疾病的致病原因，包括霍乱、炭疽和白喉。每年，这些疾病影响全世界数十万人，就炭疽病而言，还影响牲畜。面包酵母，酿酒酵母，也可以受到蛋白质毒素的影响，这种毒素使用与上述具有重要医学意义的毒素相同的细胞进入途径。这项研究将描述一种名为K28的酵母毒素的特征，以更好地了解毒素与细胞之间的一般相互作用。通过与酵母资源中心的合作，我们将能够绘制出与两个K28毒素亚基相互作用的宿主蛋白。这些信息将有助于澄清毒素如何与细胞结合，如何进入细胞，以及最终毒素如何发挥其致命作用。此外，这项工作将阐明酵母毒素可能在多大程度上成为了解毒素对人类健康和疾病重要的良好模型系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microbial toxins account for the pathology of many serious diseases including cholera, anthrax, and diphtheria. Each year these diseases impact hundreds of thousands of people worldwide and in the case of anthrax, livestock. Baker's yeast, Saccharomyces cerevisiae, can also be afflicted by protein toxins that use the same cellular route of entry as the medically significant toxins mentioned above. This research will characterize a toxin that afflicts yeast, called K28, in order to gain a great understanding of toxin-cell interactions in general. By collaborating with the Yeast Resource Center we will be able to map the host proteins with which the two K28 toxin subunits interact. This information will help to clarify how the toxin binds to cells, how it enters cells and, ultimately, how the toxin exerts its lethal effects. Moreover, this work will clarify to what extent yeast toxins might be a good model system for understanding toxins important for human health and disease.