MODEL OF PI3K/RHO-FAMILY GTPASE INTERPLAY DURING FIBROBLAST SPREADING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在我们实验室现有和计划中的成像实验的成像数据的推动下，通过细胞迁移联盟的合作，我们正在全细胞水平上建立PI3K/RFG共同调控的空间模型。在活细胞成像实验分析的指导下，将构建信号-突起耦合的唯象模型。在可能的范围内，我们将在虚拟细胞环境中开发模型或其模块。Haugh小组之前在我们的梯度传感工作中利用了Vcell的新的膜扩散能力，这些模型对其他Vcell用户公开可用。细胞机械建模功能目前由VCell团队与CMC建模计划合作开发，一旦可用，将在该项目中使用。这些将取决于下一个NrCAM项目期计划的椭圆求解器。然后，新提出的多物理层和插件架构将促进将力学纳入VCell框架。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Driven by imaging data from existing and planned imaging experiments in our laboratory and through collaborations within the Cell Migration Consortium, we are building a spatial model of PI3K/RFG co-regulation at the whole-cell level. A phenomenological model of signaling-protrusion coupling will be constructed, guided by analysis of live-cell imaging experiments. To the extent possible, we will develop the model, or modules thereof, in the Virtual Cell environment. The Haugh group has previously made use of the new membrane diffusion capabilities of VCell in our work on gradient sensing, and these models are publicly available to other VCell users. Cell mechanical modeling capabilities, currently under development by the VCell team in collaboration with the CMC modeling initiative, will be utilized in this project as they become available. These will depend on the elliptic solver planned for the next NRCAM project period. Incorporation of mechanics into the VCell framework will then be facilitated by the newly proposed multiphysics layer and plug-in architecture.