NUCLEOCYTOPLASMIC TRANSPORT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 蛋白质和核酸的核质运输对细胞的正常运作至关重要。活细胞中的荧光相关光谱(FCS)正被用来直接测量Importinβ、Importin Alpha、NTF2和RAN的反应速率和流动分数。FCS数据提供了每种核运输蛋白的基本定量参数(浓度、扩散系数)，这些参数正在被纳入核运输系统的更准确的虚拟细胞模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nucleocytoplasmic transport of proteins and nucleic acids is crucial for normal cell functioning. Fluorescence correlation spectroscopy (FCS) in live cells is being used to directly measure reaction rates and mobile fractions of components of importin beta, importin alpha, NTF2 and Ran. The FCS data provides essential quantitative parameters (concentrations, diffusion coefficients) for each of the nuclear transport proteins that are being incorporated into a more accurate Virtual Cell model for the nuclear transport system.