A PROTEOMICS RESEARCH RESOURCE FOR INTEGRATIVE BIOLOGY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本年度报告的具体目标1侧重于与现场不对称波形离子迁移率光谱分析仪相关的发展。 FAIMS在具体目标1下的努力侧重于开发方法，以提高高通量测量的灵敏度，便利对非常小的生物样本进行蛋白质组学分析。该中心的许多合作研究项目都面临着与极其有限的样本量有关的挑战，特别是那些寻求对人类活检样本进行蛋白质组学分析的项目。这一点在D.Smith的脑音素研究和M.Katze对人类肝脏活检的分析中最为明显。其他项目(Cuschieri、Moerman、Kulkarni和Warren)也将继续推动这一领域的发展需求。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific Aim 1 for this annual report focuses on developments associated with field asymmetric waveform ion mobility spectrometry analyzers. The FAIMS efforts under Specific Aim 1 focused on the development of methods to improve the sensitivity of high throughput measurements facilitating proteomic analyses of very small biological samples. Many of the Center's collaborative research projects present challenges related to extremely limited sample size, particularly those seeking proteomic analysis of human biopsy samples. This was most strikingly evident in the brain voxelation studies of D. Smith and the analyses of human liver biopsies done with M. Katze. Other projects (Cuschieri, Moerman, Kulkarni, and Warren) will also continue to drive the need for developments in this area.