THE FABRICATION AND USE OF ULTRAMICROELECTRODE SENSORS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 化学选择性超微电极构成了资源技术的支柱和我们用户群的支持。 广义上，这些电极可以分为基于离子选择性液膜的电位测定法和基于金属或碳界面处的氧化还原化学的电流测定法。 酶辅助电极在单独的项目中处理。 尽管电位传感器的发展已经朝着简单的一次性离子选择性微电极取得了重大进展，但电流型传感器的发展却不能如此。 这是一种基于金属（金、铂）或碳芯的固态传感器设计。 最终尖端直径约为4微米。 建设一直是繁琐，耗时，与可变的最终产品。 这些传感器没有商业来源。 到目前为止，BRC已经开发出用于氧气、一氧化氮、抗坏血酸盐和过氧化氢的基本电流传感器。 相同的机构用于酶辅助，其中包括葡萄糖，谷氨酸盐和乳酸盐。 提高固态微电极的制造效率是一个正在进行的项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Chemically selective ultramicroelectrodes form the backbone of the resource technology and the support of our user group. Broadly these electrodes can be divided into potentiometric, based on ion selective liquid membranes, and amperometric, based on redox chemistry at a metal or carbon interface. Enzyme assisted electrodes are dealt with in a separate project. Whereas the development of the potentiometric sensor has made significant progress towards a simple disposable ion selective microelectrode, the same cannot be said of the amperometric variety. This is a solid-state sensor design based on a metal (gold, platinum) or carbon core. The final tip diameters are approximately 4 microns. Construction has been tedious, time consuming, with a variable final product. There are no commercial sources for these sensors. To date the BRC has developed, to application, basic amperometric sensors for oxygen, nitric oxide, ascorbate and hydrogen peroxide. The same bodies are used for enzyme assisted, which cover glucose, glutamate and lactate. Improving the efficiency of fabrication of the solid-state microelectrodes is an ongoing project.