Development of Practical Mid-infrared Spectroscopic Imaging Technology for Cancer

描述(由申请人提供)：临床实践中迫切需要能够帮助做出准确、客观和自动化的决定的技术，以帮助病理学家提高准确性和控制成本。该项目的主要目标是为临床和研究提供一种实用的成像仪器，该仪器可以由病理实验室中任何训练有素的人员操作。该方法首先基于为稳健的离散频率红外(DF-IR)光谱成像开发新的仪器和分析方法。这件乐器与目前的技术水平大相径庭。其次，该方法通过先进的控制算法实现了非制冷探测器的集成，从而使该方法变得实用。最后，将开发的仪器在临床环境中进行测试，并将获得将导致临床试验的结果。如果该项目成功，它有可能改变患者的决策，并改变研究中组织学评估的标准做法。我们特别针对两个领域：快速检测前列腺癌的重要活检切片(那些诊断有效且含有癌症的切片)和快速检测乳腺癌组织中的癌症。这两种方法都是在没有任何染料、污渍或人工监督的情况下完成的。 公共卫生相关性：该项目的第一个目标是提供一种新的化学成像仪器，用于临床和研究用途，可以由病理实验室中任何训练有素的人员操作。该项目有可能改变患者的诊断和治疗决策，使其能够在没有染料、染色或人工输入的情况下做出早期决定和准确诊断。提议开发乳腺癌和前列腺癌的过程，这项技术每年可能会影响200多万癌症筛查呈阳性的人。如果成功，这里仪器和分析方法的建立将改变组织学评估未来癌症研究的标准做法。

DESCRIPTION (provided by applicant): Technologies that can help make accurate, objective and automated decisions to aid the pathologist are sorely needed in clinical practice to improve accuracy and contain costs. The major goal of this project is to provide a practical imaging instrument for clinical and research use that can be operated by any trained person in pathology laboratories. The approach is based, first, on developing novel instrumentation and analytical methods for robust discrete frequency infrared (DF-IR) spectroscopic imaging. The instrument presents a departure from the current state of the art. Second, the approach is made practical by enabling the incorporation of uncooled detectors via advanced control algorithms. Last, the developed instrument is tested in a clinical setting and results that will lead to clinical trials will be obtained. If the project is successful, it has the potential to transform decision-making for patients and alter the standard practice of histologic assessment in research. We specifically target two areas: the rapid detection of important biopsy slides in prostate cancer (those that are diagnostically valid and contain cancer) and rapid detection of cancer in breast tissues. Both are accomplished without any dyes, stains or human supervision. PUBLIC HEALTH RELEVANCE: The first goal of this project is to provide a new chemical imaging instrument for clinical and research use that can be operated by any trained person in pathology laboratories. The project has the potential to transform diagnosis and therapy decisions for patients by enabling early decisions and accurate diagnoses without dyes, stains or human input. Proposing to develop the processes for breast and prostate cancer, the technology could impact over 2 million people screened positive for cancer every year. If successful, establishment of the instrumentation and analytical methods here would alter the standard practice in histologic assessment of future research in cancer.