CHARACTERIZATION OF INOSITOL PHOSPHORYLCERAMIDES FROM LEISHMANIA MAJOR BY TANDEM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们发展了串联质谱学方法，包括多级离子陷阱和源碰撞激活解离(CAD)串联质谱仪与电喷雾电离(ESI)，以负离子模式表征肌醇磷酰神经酰胺(IPC)物种为[M-H]-和[M-2H+Li]-离子，以及以正离子模式表征为[M+H]+、[M+Li]+和[M-H+2Li]+离子(3)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed tandem mass spectrometric approaches, including multiple stage ion-trap and source collisionally activated dissociation (CAD) tandem mass spectrometry with electrospray ionization (ESI) to characterize inositol phosphorylceramide (IPC) species as [M - H]- and [M - 2H + Li]- ions in negative-ion mode and as [M + H]+, [M + Li]+, and [M - H + 2Li]+ ions in positive-ion mode (3).