Molecular and Cellular Biology Core

分子和细胞生物学（MCB）核心将为ONPRC的U 54研究人员和科学工作人员提供支持分子生物学和细胞培养的服务，并支持NICHD支持的项目。MCB Core的目标是提供灵活、省时和经济的服务，以促进研究者对分子生物学和细胞培养的利用。分子生物学服务包括生成猴特异性cDNA;自动测序;大规模质粒和噬菌体制备;提供有限数量的试剂，培训和获得 专业设备包括液体处理机器人、实时PCR和荧光成像。细胞生物学服务包括培养基和试剂的制备、细胞系的储存和扩增、包被培养器皿和盖玻片的制备、细胞系的转染和克隆、培训以及获得包括荧光板读数器在内的专用设备。MCB核心将是一个 开放核心，将45%的核心资源用于3个U 54项目，其余资源用于7个U 54批准的ROI

The Molecular and Cellular Biology (MCB) core will provide services in support of molecular biology and cell culture for U54 investigators and scientific staff at the ONPRC with NICHDsupported projects. The goal of the MCB Core is to provide flexible, time- and cost-effective services to facilitate investigator's utilization of molecular biology and cell culture. Molecular biology services include generation of monkey specific cDNAs; automated sequencing; large scale plasmid and phage preparation; supply of a limited number of reagents, training and access to specialized equipment including a liquid-handling robot, realtime PCR and phosphorimaging. Cell biology services include preparation of media and reagents, storage and amplification of cell lines, preparation of coated culture ware and cover slips, transfection and cloning of cell lines, training, and access to specialized equipment including a fluorescent plate reader. The MCB Core will be an open core dedicating 45% of core resources to 3 of the U54 projects and the remaining resources to 7 U54-approved ROls