WEST NILE VACCINE FOR NON-HUMAN PRIMATES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 本项目的目的是测试一种候选的水泡性口炎疫苗，用于保护恒河猴免受西尼罗河病毒（WNV）感染。 在TNPRC，发现36%的户外非人灵长类（NHP）群体对西尼罗河病毒呈抗体阳性。 对商店样本的比较表明，这些动物在2002年被感染。 这些数据表明，疫苗将有助于保护NHP菌落免受感染。 六只印度恒河猴被分配到这个项目。 2006年对两只动物进行了西尼罗河病毒IV接种，并于2007年再次进行了攻毒。 在接种前和接种后采集血样，使用RT-PCR和抗体测定监测疾病进展。 从两只动物中采集CSF，从一只动物中采集淋巴结活检。监测对西尼罗河病毒感染的免疫应答。 对于该项目的疫苗接种攻毒组，4头动物于2007年5月接种疫苗，30天后加强免疫。30天后对所有4只动物进行攻毒。 虽然检测到抗体，但恒河猴中WNV的低致病性排除了疫苗效力的评价。 VSV载体在小鼠中的结果已发表。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to test a candidate vesicular stomatitis vaccine for protection of rhesus macaques against West Nile Virus (WNV) infrection. At the TNPRC 36% of the outdoor non-human primate (NHP) colony were found to be antibody positive for WNV. Comparisons of store samples indicated that the animals had been infected in 2002. These data indicate that a vaccine would be helpful in protecting NHP colonies from infection. Six Indian rhesus macaques were assigned to this project. Two animals were inoculated IV with WNV in 2006 and re-challenged in 2007. Blood samples were collected prior to and post inoculation to monitor disease progression using RT-PCR and antibody assays. CSF was collected from both animals, lymph node biopsies were collected from one animal. The immune response to WNV infection was monitored. For the vaccination challenge arm of the project, four animals were vaccinated in May of 2007 and boosted 30 days later. All four animals were challenged on 30 days later. Although antibody was detected, the low pathegenecity of WNV in rhesus precluded an evaluation of vaccine efficacy. Results with VSV-vector in mice were published.