MONOLITHIC CAPILLARY-HPLC & INTRODUCTION OF INTACT PROTEINS & PROTEIN DIGEST T

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的目的是优化QSTAR-XL仪器的整体毛细管液相色谱的使用，以便在飞摩尔水平上常规地完成蛋白质的净化和完整分子量的成功测量。与C4等其他固定相的性能比较正在进行中。 预计我们将探索制造我们自己的整体式柱子，其性能优化以满足我们的研究需要。 (在协作项目和其他技术研究和开发项目下报告的额外工作量和仪器时间。)

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to optimize use of monolithic capillary liquid chromatography with the QSTAR-XL instrument so that clean-up of proteins and successful measurements of intact molecular weights can be accomplished routinely at the femtomole level. A comparison of performance with other stationary phases such as C4 is in progress. It is anticipated that we will explore fabrication of our own monolithic columns with properties optimized for our research needs. (Additional effort and instrument time reported under Collaborative projects and other Technical Research and Development projects.)