LOW LIGHT AND LUMINESCENT DETECTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 作为创建集成实验平台的驱动力的一部分，我们将建立和开发技术结合在一起，我们的目标是将低光和发光成像纳入BRC。 对于发光，我们靶向ATP的荧光素酶/荧光素酶测定。 这款没有商业来源的成像仪的成功将对几个对新陈代谢感兴趣的用户项目产生直接影响。 为了观察细胞群，我们还试图开发一种更简单但有效的光子计数系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of the drive to create integrated experimental platforms bringing together established and developing techniques we made it a goal to incorporate low light and luminescent imaging within the BRC. For luminescence we are targeting the luciferin/luciferase assay for ATP. Success with this imager, for which there are no commercial sources, will have an immediate impact on several user projects interested in matters of metabolism. For looking at populations of cells we have also sought to develop a simpler but effective photon counting system.