MEMBRANE FLUCTUATIONS OF RED BLOOD CELLS WITH DIFFRACTION PHASE MICROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 人红细胞（RBC）膜的膜是一种复合结构，提供了细胞形态和力学的主要控制。它由连接到2D血影蛋白网络的流体脂质双层组成，该网络决定了复合膜的弹性。这种结构的动力学性质影响RBC在循环中运输氧的能力。目前用于评估红细胞粘弹性的技术依赖于施加外部载荷，并且在获得宽频率范围内的动态信息方面受到限制。我们应用非接触式光学干涉技术来量化红细胞膜在很宽的空间和时间频率范围内的热波动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The membrane of a human red blood cell (RBC) membrane is a composite structure that provides the principal control of both the cell's morphology and mechanics. It consists of a fluid lipid bilayer tethered to a 2D spectrin network, which determines the composite membrane's elasticity. The dynamic properties of this structure influence the ability of RBCs to transport oxygen in circulation. Current techniques for assessing the viscoelasticity of RBCs rely on applying external loads and are limited in obtaining dynamic information over a wide range of frequencies. We apply a non-contact optical interferometric technique to quantify the thermal fluctuations of RBC membranes over a broad range of spatial and temporal frequencies.