BINGE ETHANOL AND OOCYTE QUALITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的总体目标是评估酗酒对卵母细胞质量的影响。据我们所知，还没有研究过过度饮酒对女性或任何动物模型生育能力的影响，但过度饮酒是一个日益严重的公共健康问题。在这个项目中，年轻的成年雌性恒河猴将接受训练，按照模拟女性酗酒的时间表饮酒。将评估至少6个月的狂欢酒精对卵子健康和卵巢功能的影响，包括两个周期的超排以获得进一步研究的卵母细胞。随后，雌性将进行时间交配，一旦检测到怀孕，所有乙醇消费将被停止，因此这些研究的重点将只是乙醇对卵母细胞和植入前胚胎的影响，而不是胎儿。将评估激素谱、卵母细胞发育潜力、卵母细胞和颗粒细胞及基因表达、胚胎发育以及胎儿和婴儿发育。此外，卵母细胞和颗粒细胞将在体外暴露于乙醇中，以进一步研究其作用机制。通过利用恒河猴，这些实验的结果将与女性酗酒的影响直接相关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal of this project is to evaluate the effects of binge-type alcohol exposure on oocyte quality. There are no studies known to us that have examined the effects of binge alcohol consumption on fertility in women or any animal model, yet binge drinking is an increasing public health concern. In this project, young adult female rhesus monkeys would be trained to drink alcohol on a schedule that simulates binge drinking in women. The effects of at least 6 months of binge ethanol on oocyte health and ovarian function will be evaluated, including two cycles of superovlation to obtain oocytes for further study. Subsequently, females will be time-mated and as soon as pregnancy is detected, all ethanol consumption would be discontinued so that only the effects of ethanol on the oocytes and pre-implantation embryos, not the fetus, will be the focus of these studies. Hormone profiles, potential for oocyte development, oocyte and granulosa cell and gene expression, embryo development and fetal and infant development will be evaluated. Additionally, oocytes and granulosa cells will be exposed to ethanol in vitro to further study mechanisms of action. By utilizing the rhesus monkey, the outcome of these experiments will have direct relevance to the effects of binge drinking in women.