Molecular Multispectral Imaging

描述（由申请人提供）：IVIS（R）光谱成像系统（Caliper Life Sciences，Inc.）要求，并将在罗切斯特大学医学中心（URMC）建立一个共享的仪器核心设施。在生物医学研究中，允许在不同光谱波段进行非混合数据采集的成像技术越来越有用。所要求的IVIS Spectrum具有独特的能力，可以在同一动物体内对生物发光和荧光报告分子进行灵敏成像，而不会混合多个光谱。该系统执行落射照明和透射照明荧光成像，并使用高效窄带通滤波器与光谱解混算法相结合，以区分多个浅荧光源和深荧光源。这在最佳地实现广泛的荧光/生物发光研究应用中是有用的。该系统还能够对生物发光和荧光进行3D断层重建，并能够与CT和MRI等互补模式进行数据配准。共享的仪器将补充和促进URSMD研究人员可用的广泛成像资源，并将实现新的研究方向，最终提高取得突破性医学发现和吸引大量研究赠款的前景。这项变革性技术还将使URSMD研究人员能够以更小的样本量进行受试者内、纵向、体内成像和数据采集，这将显著降低研究成本，并提高利用该仪器进行研究的统计能力。在此拨款申请中确定的用户池吸引了来自11个不同URSMD部门的十几名NIH资助的研究人员，他们从事广泛的生物医学研究领域，重点是与URSMD临床和转化科学研究所（CTSI）的使命一致的临床转化领域。这些项目包括研究各种疾病模型和治疗方法的发展，在体内和真实的时间解剖细胞和分子信号传导机制，以及组织工程和再生医学，仅举几例。URSMD明确承诺为建立拟议的分子多光谱成像设施提供空间和资源，这将有助于创造符合《美国复苏和再投资法案》目标的短期和长期就业机会。 公共卫生相关：要求为IVIS（r）光谱多模式（多光谱生物发光和荧光）成像仪器提供资金。共享的仪器将补充和促进广泛的成像资源提供给URSMD研究人员从事广泛的生物医学研究领域，重点是临床翻译与URSMD的临床和转化科学研究所（CTSI）的使命一致。

DESCRIPTION (provided by applicant): An IVIS(R) Spectrum Imaging system (Caliper Life Sciences, Inc.) is requested and will be set up as a shared instrument core facility at the University of Rochester Medical Center (URMC). Imaging technologies that allow for unmixed data acquisition at different spectral bands are increasingly useful in biomedical research The requested IVIS Spectrum has unique capabilities for sensitively imaging both bioluminescent and fluorescent reporters within the same animal without mixing the multiple spectra. The system performs both epi- and trans-illumination fluorescent imaging and uses high efficiency narrow band- pass filters coupled with spectral unmixing algorithms to differentiate between multiple shallow and deep fluorescent sources. This is useful in optimally enabling a wide array of fluorescence/bioluminescence research applications. The system is also capable of performing 3D tomographic reconstructions for BOTH bioluminescence and fluorescence, and enables data co-registration with complementary modalities such as CT and MRI. The shared instrument will compliment and boost the extensive imaging resources available to URSMD researchers and will enable new research directions that will ultimately enhance the prospects of making breakthrough medical discoveries and attracting substantial research grants funding. This transformative technology will also allow URSMD researchers to perform intra-subject, longitudinal, in vivo imaging and data acquisition with significantly smaller sample sizes, which will significantly reduce the cost of research and improve statistical power of the studies utilizing this instrument. The User's pool identified in this grant application draws a dozen NIH-funded investigators from 11 different URSMD departments pursuing wide ranging areas of biomedical research with emphasis on clinical translational areas that are well-aligned with the mission of the URSMD's Clinical and Translational Sciences Institute (CTSI). These projects include studying various disease models and development of therapeutics, dissecting cellular and molecular signaling mechanisms in vivo and in real time, and tissue engineering and regenerative medicine, to name a few. The URSMD has made an unequivocal commitment to the provision of space and resources towards the establishment of the proposed Molecular Multispectral Imaging facility, which will help generate short-term and long-term employment opportunities that are consistent with the goals of the American Recovery and Reinvestment Act (ARRA). PUBLIC HEALTH RELEVACNE: Funding for an IVIS(r) Spectrum Multimodal (multispectral bioluminescence and fluorescence) Imaging instrument is requested. The shared instrument will compliment and boost the extensive imaging resources available to URSMD researchers engaged in wide ranging areas of biomedical research with emphasis on clinical translation in alignment with the mission of the URSMD's Clinical and Translational Sciences Institute (CTSI).