REQUEST TO PURCHASE A VARIAN HIGH RESOLUTION NMR REPLACEMENT CONSOLE

描述（由申请人提供）：华盛顿大学高分辨率核磁共振设施请求资助购买用于老化500 MHz核磁共振仪器的Varian DirectDriveT控制台和魔角样本旋转NanoprobeT，以研究癌症组织的代谢物谱。这些请求是为了补充和现代化共享的磁共振资源，该资源为38个独立的研究小组提供服务，这些研究小组包括麻醉学，生物化学和分子生物物理学，化学，心脏病学，工程学，内科学，神经病学，眼科学，病理学，药理学和分子生物学，物理学，放射肿瘤学和放射学。NMR设施是一个活跃的资源，拥有五种仪器，并配备了致力于用户培训和合作研究的经验丰富的专业人员。该设施全年365天、每天24小时开放，致力于为其广泛的用户群提供服务。提出这一要求是因为现有的设施500 MHz仪器可追溯到1986年，其停机时间长，维护费用高。二十多年来，该仪器一直为数十名主要研究人员的研究需求服务，现在已经过时，不可靠。该建议的科学依据涵盖了长期关注健康相关问题的用户群体。这些包括光引发的DNA结构改变的研究领域，模拟神经通讯网络的分子结构的创建，理解癌症的起源和识别的标记，活性氧化分子在生命系统中引起的组织损伤，以及理解糖尿病心脏的代谢改变。这项314 395美元的请求得到了华盛顿大学75 000美元的对等资金承诺。

DESCRIPTION (provided by applicant): The Washington University High Resolution NMR Facility requests funding to purchase a Varian DirectDriveT console for an aging 500 MHz NMR instrument and a magic angle sample spinning NanoprobeT to allow for studies of metabolite profiles from cancer tissues. These requests are for supplementing and modernizing a shared magnetic resonance resource that serves 38 separate research groups spanning departments of Anesthesiology, Biochemistry & Molecular Biophysics, Chemistry, Cardiology, Engineering, Internal Medicine, Neurology, Ophthalmology, Pathology, Pharmacology & Molecular Biology, Physics, Radiation Oncology, and Radiology. The NMR Facility is an active resource housing five instruments and staffed with seasoned professionals committed to user training and collaborative research. The Facility is open and available 24 hours a day, 365 days a year and committed to service for its extensive user base. This request is prompted by significant down time and maintenance expense associated with the existing Facility 500 MHz instrument that dates back to 1986. This instrument has served research needs for dozens of principal investigators for over two decades and is now dated and unreliable. The scientific basis for this proposal spans a user group with longstanding interests in health related issues. These including research areas of light initiated alterations of DNA structure, the creation of molecular structures that mimic neural communication networks, understanding markers for the genesis of and recognition of cancer, tissue damage caused by reactive oxidative molecules in living systems, and understanding the altered metabolism of the diabetic heart. This request, $314,395, is made with a financial matching commitment from Washington University in the sum of $75,000.