HIGHLY EFFECTIVE CONTROL OF AIDS VIRUS CHALLENGE IN MACAQUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在这个方案中，18只猕猴被分配到三组中的一组。分别于2009年4月、6月和8月用空载体(对照)、VSV/SFV E660或VSV/SFV E660+GMCSF免疫动物。所有动物在2009年9月接受了SIVsmE660的挑战。 接种对照疫苗的6只动物全部被感染，最高血浆病毒载量在6.0到8.2个对数拷贝/毫升之间。三个对照动物的PVL在攻击后稳步下降(其中两个目前低于可检测的限度)，两个对照动物的PVL保持在6.0到7.0个对数拷贝/毫升之间。最后，一个对照动物因神经功能障碍而被人道安乐死。功能障碍的病因尚不清楚(组织学尚未确定)。 在接受VSV/SFV E660+GMCSF的六只动物中，有五只感染了峰值PVL，范围在4.7到7.8个对数拷贝/毫升之间。其中一只动物目前的PVL低于可检测的限度，这组中的另一只动物的PVL在攻击后稳步下降。3只动物的PVL保持在4.0-6.0log Copies/ml之间。 接种VSV/SFV E660的6只动物中有2只感染峰值PVL分别为3.9和5.8log拷贝/ml，在攻毒后第35天和42天，这两只动物的PVL均恢复到检测不到的水平。 所有动物将继续接受监测，以跟踪感染或缺乏感染的进展情况。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Eighteen Rhesus macaques are assigned to one of three groups on this protocol. Animals were immunized with either empty vectors (controls), VSV/SFV E660 or VSV/SFV E660 + GMCSF in April, June and August 2009. All animals were challenged with SIVsmE660 in September 2009. All six animals receiving control vaccines became infected, with peak plasma viral loads ranging between 6.0 and 8.2 log copies/ml. PVL of three control animals has steadily declined after challenge (two of which are currently below detectable limits) and PVL of two control animals remains high between 6.0 and 7.0 log copies/ml. Finally one control animal was humanely euthanized due to neurologic dysfunction. The etiology of the dysfunction is not yet known (histology is pending). Five of the six animals receiving VSV/SFV E660 + GMCSF became infected with peak PVL ranging between 4.7 and 7.8 log copies/ml. One of these animals currently has PVL below detectable limits and the PVL of another animal in this group has steadily declined after challenge. Three animals exhibit PVL remaining steady between 4.0 and 6.0 log copies/ml. Two of six animals receiving VSV/SFV E660 became infected with peak PVL of 3.9 and 5.8 log copies/ml. The PVL of both of these animals returned to undetectable levels by day 35 and 42 post-challenge. All animals will continue to be monitored to follow the progression of infection or lack thereof.