CONFOCAL MICROSCOPY AND IMAGE ANALYSIS CORE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该核心为TNPRC的每个部门以及几个机构的众多附属研究科学家提供最先进的共聚焦显微镜、多标记荧光标记和检测以及图像分析支持。 Core配备了Leica TCS SP2激光扫描共聚焦显微镜系统，配备3个激光器，有6条激光线可用，能够同时收集四个通道的信息（3个荧光通道和一个微分干涉对比通道）。 该系统连接到两个显微镜，一个直立（DMRE）和一个倒置（DMIRE 2），允许固定制剂和活细胞的共聚焦显微镜。我们有一个单独的工作站来运行Leica软件，以分析收集的数据。我们也有Volocity软件用于三维数据的渲染。透射光图像分析系统基于Leica DMRE显微镜、Spot摄像头和最新的ImagePro软件，在专用工作站中供研究人员用于图像分析。a）多维成像，因为可以获得四个维度的图像，即长度（x轴）、宽度（y轴）、深度（z轴）和时间（t）。 B）分辨率提高。c）对比度改善。d）多色成像。 几种荧光染料可以同时成像。 在过去的一年里，四个新用户接受了培训，并获准在没有帮助的情况下使用共焦显微镜。 此外，核心人员还在180个时段、750个小时内协助其他用户。 该核心执行了300项成像服务，包括体内成像和2或3色显微镜，产生了超过400千兆字节的数据。 核心直接为4份新出版物提供了捐助。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides state-of-the-art confocal microscopy, multilabel fluorescent labeling and detection, and image analysis support to every Division in the TNPRC, and to numerous affiliate research scientists at several institutions. The Core has a Leica TCS SP2 laser scanning confocal microscope system equipped with 3 lasers, with 6 laser lines available, capable of simultaneously collecting information in four channels (3 fluorescent and one for differential interference contrast). The system is attached to two microscopes an upright (DMRE) and an inverted (DMIRE2), that allow for confocal microscopy of fixed preparations and also living cells. We have a separate workstation to run the Leica software for the analysis of the data collected. We also have Volocity Software for the rendition of the data in 3 dimensions. The Image analysis system for transmitted light is based in the Leica DMRE microscope, a Spot camera and the latest ImagePro software in a dedicated workstation for investigators use in image analysis. The confocal system offers such benefits as: a) Multi-dimensional imaging, as it is possible to obtain images in four dimensions, length (x axis), width (y axis), depth (z axis) and time (t). b) Resolution improvement. c) Contrast improvement. d) Multicolor imaging. Several fluorochromes can be imaged at the same time. In the last year four new users were trained and approved to use the confocal microscope without assistance. In addition, core personnel assisted other users in 180 timeslots for 750 hours. The core performed 300 imaging services, including in vivo imaging and 2 or 3 color microscopy that generated more than 400 gigabytes of data. The core directly contributed to 4 new publications.