PRIMATE PLURIPOTENT CELLS FOR AUTOLOGOUS TRANSPLANTATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项建议的目的是研究成年体细胞重编程实验诱导恒河猴多能细胞的潜力，方法有两种：体细胞核移植(SCNT)和直接重编程。在这项应用中，我们将从成年猴子皮肤成纤维细胞中产生基因相同的SCNT-ES和iPS细胞，并使用一系列广泛的测试将它们相互比较，并与从受精胚胎产生的ES细胞进行比较，包括产生嵌合体的能力，最终验证它们的多能性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this proposal is to investigate the potential of rhesus monkey pluripotent cells experimentally induced by reprogramming of adult somatic cells using two alternative approaches: somatic cell nuclear transfer (SCNT) and direct reprogramming. In this application, we will generate genetically identical SCNT-ES and iPS cells from adult monkey skin fibroblasts and compare them to each other and to ES cells produced from fertilized embryos using an array of extensive tests including the ability to generate chimeras, the ultimate validation of their pluripotency.