IDENTIFICATION OF DRUG TARGETS FOR STIMULATING OXYTOCIN RELEASE IN NHP BRAIN

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在报告期间，我们记录了几十个大鼠SON和PVN神经元，然后提取它们的mRNA用于RT-PCR。 这种方法使我们能够识别OT神经元与其他神经元相比的电生理特征。 我们也一直在筛选血清素受体和其他神经肽受体系统在这些神经元中使用RT-PCR。 我们还有机会记录机会收集的灵长类动物大脑样本，并希望分析它们的mRNA中的受体mRNA。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During the reporting period, we have recorded from several dozen SON and PVN neurons from rats, then pulled their mRNA to use for RT-PCR. This approach has allowed us to recognize the electrophysiological signature of OT neurons compared to other neurons. We have also been screening for serotonin receptors and other neuropeptide receptor systems in these neurons using RT-PCR. We have also had the opportunity to record from primate brain samples collected opportunistically and hope to analyze their mRNA for receptors mRNAs as well.