Tissue procurement & processing for molecular analysis

描述（由申请人提供）：本提案的目的是继续中大西洋分部的努力，根据NCI支持的生物储存库的第一代指南，为合作人体组织网络的用户提供高质量的人体组织和生物流体样本。组织样本通过组织学检查进行质量控制，生物样本的分子评估包括在质量控制程序中。如有可能，将征求患者知情同意，并按照批准的豁免同意指南的规定，制定程序对生物标本进行匿名处理。私人健康信息按照通用规则和HIPAA法规处理。为保护受试者隐私，所有样本仅通过代码编号识别（“去识别”），链接代码保存在安全数据库中。采购的组织标本以新鲜冷冻、化学固定、石蜡包埋和活的形式提供。活组织的选择包括来自机械和酶促分解的固体组织的冷冻细胞悬浮液、小组织碎片的冷冻细胞悬浮液、从淋巴结分解的淋巴细胞的冷冻细胞悬浮液和从腹水获得的活细胞的冷冻细胞悬浮液。除石蜡包埋外，还提供石蜡包埋或冷冻组织的组织切片。组织学引导的宏观解剖服务可用于需要富集细胞群的研究者。将为散装和宏观解剖组织样本提供核酸提取（RNA和DNA）。CHTN组织微阵列（TMA）计划主要基于中大西洋分部，将继续得到支持并将扩大。我们机构提供注释数据的能力将通过与弗吉尼亚大学的数字临床数据库和已建立的肿瘤登记处相结合来增强。在资助期间，我们部门的成员将合作整合当地的信息资源与集中的CHTN信息资源，并实现符合CABIG或其他NCI授权的生物信息标准。中大西洋分部的成员将通过参加协调会议和科学会议的营销工作来支持CHTN的整体努力。CHTN的改进将通过参与组织质量管理实践和在整合新技术和生物信息学进步方面提供领导来提供。

DESCRIPTION (provided by applicant): The objectives of this proposal are to continue the efforts of the Mid-Atlantic division to provide users of the Cooperative Human Tissue Network with high quality human tissue and biofluid samples in accordance with the first generation guidelines for NCI-supported biorepositories. Tissue samples are quality controlled by histologic examination, with molecular assessment of biospecimens included in quality control procedures. Informed patient consent will be sought when possible and procedures are in place to anonymize biospecimens as indicated by approved waiver of consent guidelines. Private health information is handled in accordance with Common Rule and HIPAA regulations. To protect subject privacy, all samples are identified only by a code number ("de-identified") and linking codes are maintained in secure databases. Procured tissue specimens are made available in fresh-frozen, chemically-fixed, paraffin-embedded and viable form. Options for viable tissue include frozen cell suspensions from mechanically and enzymatically disaggregated solid tissue, frozen cell suspensions of small tissue fragments, frozen cell suspensions of lymphocytes disaggregated from lymph nodes and frozen cell suspensions of viable cells obtained from ascites fluid. In addition to paraffin embedding, histologic sections of paraffin embedded or frozen tissue are offered. Histology-guided macrodissection services are available to investigators who require enriched cell populations. Nucleic acid extraction (RNA and DNA) will be offered for both bulk and macrodissected tissue samples. The CHTN tissue microarray (TMA) initiative, which is predominantly based at the Mid-Atlantic division, will continue to be supported and will be expanded. The ability of our institution to provide annotated data will be enhanced by integrating with a digital clinical data repository at The University of Virginia and with an established tumor registry. During the grant period members of our division will cooperate to integrate local informatic resources with centralized CHTN informatic resources, and to achieve compliance with caBIG, or other NCI-mandated bioinformatic standards. Members of the Mid-Atlantic division will support the overall efforts of the CHTN by participating in coordinating meetings and in marketing efforts at scientific meetings. Improvement of the CHTN will be provided by participating in organizational quality management practices and offering leadership in integrating new technological and bio- informatic advances.