TULANE COBRE: MOLECULAR, IMAGING AND ANALYTIC CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子、成像和分析核心由2个交互设备组成：A)分子和成像设备，B)RIA和分析设备。 分子和成像设施拥有宝贵的工作站，可以执行以下活动：使用GeneAmp聚合酶链式反应系统对基因靶向的小鼠进行基因分型的DNA工作；使用LICO公司的奥德赛系统进行蛋白质印迹分析；使用Stratagene公司的实时荧光聚合酶链式反应系统；以及使用Qiagen公司的BioRobot EZ1和ELISA站进行各种检测的RNA提取。一个单独的组织学免疫组织化学工作室专门用于形态和定量免疫组织化学。奥林巴斯荧光显微镜配备了利用Image-Pro Plus分析系统的实时数字图像捕获系统，可以量化免疫染色的变化。在一个单独的设备中，血管紧张素肽的高效液相分离和分析与放射免疫分析相结合，提供了一种定量分析血浆、组织和尿中的血管紧张素肽和其他激素因子的方法。对于每个主要设备，都有一名研究技术员接受培训，为调查人员提供支持。然而，个别调查人员、研究员和学生也接受了各种技术方面的培训。这些设置使研究人员从复杂的操作中解脱出来，专注于他们的研究项目。 放射免疫分析和分析设施对血浆、组织和尿样进行放射免疫分析和酶联免疫吸附分析。最近的重点是人、大鼠和小鼠血管紧张素原的酶联免疫吸附试验的发展。我们开发的人类血管紧张素原酶联免疫吸附试验导致了许多人要求与其他研究人员合作，进行与原发性高血压、糖尿病和慢性肾脏疾病的肾内肾素-血管紧张素受体相关的翻译项目。利用我们核心设施的已发表研究列在出版物项下。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular, Imaging, and Analytic Core consists of 2 interactive facilities: A) Molecular and imaging facility, B) RIA and Analytic facility. The molecular and imaging facility has valuable work stations to perform the following activities: DNA work for genotyping of gene-targeted mice using the GeneAmp PCR system, Western blot analysis using Lico's Odyssey system, Stratagene's real-time PCR system and RNA extraction using Qiagen's BioRobot EZ1 and ELISA station for various assays. A separate histology immunohistochemistry work room is dedicated for morphology and quantitive immunohistochemistry. An Olympus fluorescence microscope equipped with a real time digital image capturing system utilizing the Image-Pro Plus analyzing system allows for quantization of the changes in immunostaining. In a separate facility, HPLC separation and analysis of angiotensin peptides combined with RIA provides a means for quantitive analysis of angiotensin peptides and other hormonal factors in plasma, tissues, and urine. For each major piece of equipment, a research technician is trained to provide support to investigators. However, the individual investigators, fellows, and students are also trained in the various techniques. These setups allow investigators to be free from complicated operations and to focus on their research projects. The RIA and analytic facility performs RIA and ELISA analysis of plasma, tissue, and urine samples. Recent emphasis has been on the development of ELISA for human, rat, and mouse angiotensinogen. Our development of an ELISA for human angiotensinogen has led to many requests to collaborate with other investigators on translational projects related to the intrarenal Renin-Angiotensin receptor in essential hypertension, diabetes, and chronic kidney disease. Published studies that utilized our Core facilities are listed under publications.