CELL CYCLE REGULATION OF GENE EXPRESSION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目将分析细胞周期调节蛋白对特定抑制剂的反应变化。我们将用二甲双胍等抑制剂处理正常细胞和乳腺癌细胞系，然后观察其对细胞周期蛋白D启动子和p27表达的影响。这项工作将包括分离质粒DNA、转染培养细胞和各种生化分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project will involve analysis of changes in cell cycle regulatory proteins in response to specific inhibitors. We will treat normal cells and breast cancer cell lines with inhibitors such as metformin and then observe the effects on the cyclin D promoter and p27 expression. The work will involve isolating plasmid DNAs, transfection of cultured cells and various biochemical assays.