HIGH THROUGHPUT STRUCTURE DETERMINATION AT CESG

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 真核生物结构基因组学中心(CESG)正在开发快速核磁共振筛选方法，以确定从注释的拟南芥基因组中选择的结构基因组学靶标的蛋白质稳定性、溶解性、构象状态和配体结合特性。特别强调其序列可能包含新折叠的蛋白质、与新功能相关的蛋白质、或可能具有已知折叠但具有先前未与该折叠相关的功能的蛋白质。我们将利用现有的方法，开发新的核磁共振方法来高通量地确定蛋白质的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Center for Eukaryotic Structural Genomics (CESG) is developing rapid NMR screening methods for determining protein stability, solubility, and conformational states as well as ligand binding properties of structural genomics targets selected from the annotated Arabidopsis thaliana genome. Particular emphasis is placed on proteins whose sequences suggest that they may contain a novel fold, proteins associated with novel functions, or proteins likely to have a known fold but with a function not previously associated with that fold. We will use the existing methods and develop novel NMR methods for the high throughput protein structure determination.