SPECTROSCOPIC PHASE MICROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们开发了一种实用的方法，使用样品诱导的相移光谱成像的特定分子的无标记定量。衍射相位显微术是利用不同波长的光源来记录波长相关的相位图像。我们对细胞中存在的单分子物种（如白蛋白和血红蛋白（Hb））的纯溶液进行了色散测量。利用分子特异性分散的这种先前校准，我们证明了从个体红细胞（RBC）中提取Hb浓度。本研究的终点是无创监测完整活细胞的生理状态。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We developed a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We performed dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrated the extraction of Hb concentration from individual red blood cells (RBCs). The end point of this study is non-invasive monitoring of physiological states of intact living cells.