Flow Cytometry

流式细胞术设备（FCF）是一种共享资源，为癌症中心的研究人员提供 获得高质量、高性价比的流式细胞仪服务和技术。通过提供这些服务 以及有效利用这项技术所需的科学专门知识，该设施有助于提高 在大学进行的癌症研究的范围和质量。凭借最先进的仪器， 设施提供高速4路细胞分选和克隆，复杂的细胞分析服务，多路复用 用于可溶性分析物的分析和成像流式细胞术。该仪器兼容广泛的 多种流式细胞术应用，例如亚群鉴定/定量、分子检测 （使用标记的抗体或其他配体或荧光蛋白报告分子），DNA的测量 和用于细胞周期分析的RNA含量、细胞凋亡、转录活性、细胞内离子浓度（例如， Ca++）、细胞活力、膜电位、微阵列和基于珠的免疫测定。除了有 FCF经验丰富的工作人员提供实验设计方面的咨询， 样品制备和数据分析。研究人员可以选择，一旦接受培训， 分析或利用设施的工作人员的专业知识来运行他们的样本。专门培训 课程是为那些希望更好地理解原理和技术的研究人员提供的。 他们更喜欢直接获取和/或分析他们自己的样品。的服务 FCF提供的专业知识在许多类型的癌症研究中发挥着关键作用， 血液恶性肿瘤，以及对癌症的免疫反应。

The Flow Cytometry Facility (FCF) is a Shared Resource that provides Cancer Center investigators access to high quality, cost effective flow cytometry services and technology. By providing these services and the scientific expertise necessary to effectively use this technology, the facility serves to enhance the scope and quality of cancer research performed at the University. With state of the art instrumentation, the facility offers high speed 4 way cell sorting and cloning, complex multicolor analytical services, multiplexing assays for soluble analytes, and imaging flow cytometry. This instrumentation is compatible with a wide variety of flow cytometric applications such as subpopulation identification/quantification, molecular detection (using labeled antibodies or other ligands or fluorescent protein reporter molecules), measurement of DNA and RNA content for cell cycle analysis, apoptosis, transcriptional activity, intracellular ion concentration (e.g. Ca++), cell viability, membrane potential, microarrays, and bead based immunoassays. In addition to the instrumentation, the highly experienced staff of the FCF provides consultation in experimental design, sample preparation and data analysis. Researchers have the option, once trained, of performing their own analysis or utilizing the expertise of the facility's staff to run their samples for them. Specialized training classes are offered for those researchers who wish to better understand the principles and techniques employed in this technology and prefer to directly acquire and/or analyze their own samples. The services and expertise offered by the FCF play a key role in the study of many types of cancer, especially hematological malignancies, as well as immune responses to cancers.