BINGE ETHANOL AND OOCYTE QUALITY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本项目的总体目标是评估酗酒型酒精暴露对卵母细胞质量的影响。我们还没有研究过酗酒对女性或任何动物模型生育能力的影响，但酗酒是一个日益严重的公共卫生问题。在这个项目中，年轻的成年雌性恒河猴将被训练按照模拟女性酗酒的时间表饮酒。将评估至少6个月的酒精狂欢对卵母细胞健康和卵巢功能的影响，包括两个周期的超排以获得卵母细胞用于进一步研究。随后，将对雌性动物进行定时交配，一旦检测到妊娠，将停止所有乙醇消耗，因此这些研究的重点仅是乙醇对卵母细胞和植入前胚胎的影响，而不是对胎儿的影响。将评价激素特征、卵母细胞发育潜力、卵母细胞和颗粒细胞及基因表达、胚胎发育以及胎儿和婴儿发育。此外，卵母细胞和颗粒细胞将在体外暴露于乙醇，以进一步研究作用机制。通过利用恒河猴，这些实验的结果将与女性酗酒的影响直接相关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The overall goal of this project is to evaluate the effects of binge-type alcohol exposure on oocyte quality. There are no studies known to us that have examined the effects of binge alcohol consumption on fertility in women or any animal model, yet binge drinking is an increasing public health concern. In this project, young adult female rhesus monkeys would be trained to drink alcohol on a schedule that simulates binge drinking in women. The effects of at least 6 months of binge ethanol on oocyte health and ovarian function will be evaluated, including two cycles of superovlation to obtain oocytes for further study. Subsequently, females will be time-mated and as soon as pregnancy is detected, all ethanol consumption would be discontinued so that only the effects of ethanol on the oocytes and pre-implantation embryos, not the fetus, will be the focus of these studies. Hormone profiles, potential for oocyte development, oocyte and granulosa cell and gene expression, embryo development and fetal and infant development will be evaluated. Additionally, oocytes and granulosa cells will be exposed to ethanol in vitro to further study mechanisms of action. By utilizing the rhesus monkey, the outcome of these experiments will have direct relevance to the effects of binge drinking in women.