TULANE COBRE: MOLECULAR, IMAGING AND ANALYTIC CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 分子、成像和分析核心实验室由3个房间和9个部分组成，如下所示： 1.实验所主工作间 1-1.DNA工作区 这个空间专门用于DNA工作，如基因靶向小鼠的基因分型和分析目标基因的启动子区域。一名训练有素的实验室研究技术员将处理样本。他/她将从剪下的基因靶向小鼠的尾巴中提取基因组DNA，并进行PCR。他/她还将建立琼脂糖凝胶电泳法，并将最终结果报告给相应的调查人员。主要设备包括：应用生物系统公司的GeneAmp聚合酶链式反应系统、费希尔科学公司的双室水浴、三洋的微波炉、费希尔科学公司的电源、Bio Rad的水平琼脂糖凝胶电泳仪、UVP的紫外线透光仪、费希尔科学公司的便携式离心机和New Brunswick科学公司的孵化器摇床。 博士后研究员：佐藤亮，博士 实验室研究技术员：密歇根州G.迈克·厄普丘奇 1比2。Western Blot/Gel Shift分析区域 这个空间专门用于蛋白质印迹分析/凝胶漂移分析。一名训练有素的实验室研究技术员将处理样本。他/她将从研究人员提供的人/鼠/鼠样本或细胞中提取蛋白质样本。他/她还将建立聚丙烯酰胺凝胶电泳法，并将最终结果报告给相应的调查人员。包括的主要设备有：LICOR的奥德赛系统、Pharmacia Biotech的电源和Novex的垂直寻呼系统。 博士后研究员：宫田佳子，博士 实验室研究技术员：塞勒姆·I·埃尔哈亚特，密苏里州 1比3。共享开放空间 这片空地被共享为吸液站。主要设备包括：Next Advance Inc.的S搅拌机蓝、费希尔科学公司的AcuSpin Micro17R冷冻微型离心机、丹佛仪器公司的S优质秤、Thermolyne公司的平台振荡器和费希尔科学公司的搅拌加热板。 1比4。组织学/免疫组织化学工作区 这个空间专门用于组织学/免疫组织化学工作。一名训练有素的实验室研究技术员将处理样本。他/她将设置由调查人员提供的人/鼠/鼠样本的载玻镜。他/她还将进行组织学和/或免疫组织化学染色，并将染色的玻片退回给相应的调查人员。主要设备包括：达科细胞自动染色免疫组织化学机器人，费舍尔科学公司的化学休姆罩用于过滤，费舍尔科学公司的幻灯片烤箱。 博士后研究员：神山真美博士 实验室研究技术员：塞勒姆·I·埃尔哈亚特，密苏里州 1比6。RNA工作区 这个空间专门用于RNA提取和实时RT-PCR等RNA工作。训练有素的实验室研究技术人员将处理样本。他/她将使用乔根的机器人系统从研究人员提供的人类/大鼠/小鼠样本或细胞中提取总RNA样本。他/她还将建立实时RT-PCR，并将最终结果报告给相应的调查人员。主要设备包括：Thermo Science的NanoDrop 2000、Qiagen的BioRobot EZ1、amersham Biosciences的GeneQuant光谱仪、Fisher Science的便携式离心机和Fisher Science的组织匀浆机。 博士后研究员：佐藤亮，博士 实验室研究技术员：密歇根州G.迈克·厄普丘奇 1比7。分析设备站 这个空间是专门用来放置分析设备的。主要设备有：Stratagene的实时PCR机(MX3000P和MX3005P)和Alphainotech的密度仪。 2.细胞培养工作室 一个单独的房间专门用于细胞培养。主要设备包括：Nexelom Celleter的自动细胞计数器、Labcono的II级净化器生物安全柜Heraeus的细胞孵化器、Fisher Science的倒置显微镜和Eppendorf的台式冷冻离心机。 博士后研究员：佐藤亮，博士 实验室研究技术员：密歇根州G.迈克·厄普丘奇 3.显微镜工作室 一个单独的房间专门用来放显微镜和图像分析。主要设备有：研华FL数字倒置显微镜，奥林巴斯(普通)显微镜配备实时数字图像采集系统，奥林巴斯荧光就绪显微镜配备实时数字图像采集系统，以及Image-Pro插图处理/分析系统。 博士后研究员：神山真美博士 医学研究专家：佐藤昭美，医学博士 实验室研究技术员：塞勒姆·I·埃尔哈亚特，密苏里州

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Molecular, Imaging, and Analytical Core Laboratory consists of 3 rooms and 9 parts as follows: 1. Main Laboratory Workroom 1-1. DNA Workspace This space is dedicated for DNA work such as genotyping of gene-targeted mice and analyzing for promoter regions of target genes. A well-trained laboratory research technician will handle samples. He/She will extract genomic DNA from the snipped tails of gene-targeted mice and perform PCR. He/She will also set up agarose gel electrophoresis and report the final results to the corresponding investigators. Included major equipment are: Applied Biosystems GeneAmp PCR System, Fisher Scientific's dual chamber water bath, Sanyo's microwave, Fisher Scientific's power supply, Bio Rad's horizontal agarose gel-electrophoresis system, UVP's ultraviolet transilluminator, Fisher Scientific's portable centrifuge machine, and New Brunswick Scientific's incubator shaker. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 1-2. Western Blot/Gel Shift Assay Area This space is dedicated for western blot analysis/gel shift assay. A well-trained laboratory research technician will handle samples. He/She will extract protein samplesfrom human/rats/mouse samples or cells provided by the investigators. He/She will also set up polyacrylamide gel electrophoresis and report the final results to the corresponding investigators. Included major equipments are: Licor's Odyssey system, Pharmacia Biotech's power supply and Novex's vertical PAGE system. Postdoctoral Research Fellow: Kayoko MIYATA, PhD Laboratory Research Technician: Salem I. ELKHAYAT, MS 1-3. Shared Open Space This open space is shared as a pipetting station. Included major equipment are: Next Advance Inc.'s Bullet Blender BLUE, Fisher Scientific's accuSpin Micro17R refrigerated microcentrifuge, Denver Instrument¿s high quality scale, Thermolyne's platform shaker and Fisher Scientific's stirring hotplate. 1-4. Histology/Immunohistochemistry Workspace This space is dedicated for histology/ immunohistochemistry works. A well-trained laboratory research technician will handle samples. He/She will set up slide glasses of human/rats/mouse samples provided by the investigators. He/She will also perform histology and/or immunohistochemistry staining and return the stained slides to the corresponding investigators. Included major equipment are: Dako Cytomation's Autostainer for immunohistochemistry robot, Fisher Scientific's chemical hume hood for straining, Fisher Scientific's slide oven. Postdoctoral Research Fellow: Masumi KAMIYAMA, PhD Laboratory Research Technician: Salem I. ELKHAYAT, MS 1-6. RNA Workspace This space is dedicated for RNA work such like RNA extraction and real-time RT-PCR.A well-trained laboratory research technician will handle samples. He/She will extract total RNA samples from human/rats/mouse samples or cells provided by the investigators using Qiagen's robotic system. He/She will also set up real-time RT-PCR and report the final results to the corresponding investigators. Included major equipments are: Thermo Scientific's NanoDrop 2000, Qiagen's BioRobot EZ1, Amersham Biosciences' GeneQuant spectrometer, Fisher Scientific's portable centrifuge machine and Fisher Scientific's tissue homogenizer. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 1-7. Analytical Equipment Station This space is dedicated for analytical equipments. Major equipment are: Stratagene's real-time PCR machines (MX3000P and MX3005P) and Alphainotech's densitometric machine. 2. Cell Culture Workroom A separated room is dedicated for cell cultures. Included major equipment are: Nexcelom Cellometer's Automated Cell Counter, Labconco's purifier class II biosafety cabinet Heraeus's cell incubator, Fisher Scientific's inverted microscope and Eppendorf's bench-top refrigerated centrifuge machine. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 3. Microscope Workroom A separate room is dedicated for microscopes and image analysis. Major equipment are: EVOS fl digital inverted microscope, Olympus's (general) microscope equipped to real-time digital image capturing system, Olympus's fluorescence-ready microscope equipped to real-time digital image capturing system, and Image-Pro plusimage handling/analyzing system. Postdoctoral Research Fellow: Masumi KAMIYAMA, PhD Medical Research Specialist: Akemi SATO, MS Laboratory Research Technician: Salem I. ELKHAYAT, MS