SC COBRE: LIPIDOMICS CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 脂肪组学核心正在全面运作，并正在向Cobre项目调查人员提供以下支持：(I)鞘脂化学、分析和生物学方面的专业知识，(Ii)协助实验设计和分析数据的解释，(Iii)对生物样本中的关键鞘脂进行定性和定量分析：细胞、组织、血清和介质。分析方法基于高效液相色谱-串联质谱仪(LC-MS/MS)技术。目前，CORE提供约100种不同脂类的代谢图谱，包括：鞘氨醇、二氢鞘氨醇、植脂醇及其磷酸盐、神经酰胺、α-羟基神经酰胺、二氢神经酰胺、植物神经酰胺、α-羟基植物神经酰胺及其磷酸盐、鞘磷脂、己糖-神经酰胺、乳糖基神经酰胺和二酰甘油(DAG‘s)成分。此外，还提供了用SCF-MS/MS方法单独分析葡萄糖神经酰胺和半乳糖神经酰胺分子物种的方法。(Iv)用于细胞、体外和体内研究的同源合成鞘磷脂及其类似物。目前，CORE提供修饰的狮身人面像碱基、神经酰胺及其磷酸盐、鞘磷脂、鞘磷脂新陈代谢调节剂的立体异构体，以及先进的分子探测器：功能化的、荧光的、侧特异性的放射性鞘磷脂和17C主干的鞘磷脂。 此外，Cobre研究人员还可以使用分析方法来研究鞘脂代谢和酶活性(使用由17C-主干组成的鞘脂和MS分析)。 从2010年7月到2011年4月6日，分析分核心对当前的Cobre项目进行了2612次分析(每个项目的分析下限估计为每年300次分析)，对以前的Cobre毕业生进行了477次分析：Argraves博士(87)、Hammad博士(66)、Hama博士(44)和Gudz博士(280)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Lipidomics Core is in full operation and is providing the following support to COBRE project investigators: (i) expertise in sphingolipid chemistry, analysis and biology, (ii) assistance with experimental design and interpretation of analytical data, (iii) qualitative and quantitative analysis of key sphingolipids from biological samples: cells, tissue, serum and media. Analytical approach is based on High Performance Liquid Chromatography-Tandem Mass spectrometry (LC-MS/MS) technique. Currently, core provides metabolomic profiling of ~ 100 different lipid species including: sphingosine, dihydrosphingosine, phytosphingosine and their phosphates, ceramides, alpha-hydroxy-ceramides, dihydroceramides, phytoceramides, alpha-hydroxy-phytoceramides and their phosphates, sphingomyelin, hexosyl-ceramide, lactosyl ceramide and diacyl-glycerol (DAG's) components. Additionally, a separate analysis of glucosylceramide and galactosylceramide molecular species by SCF-MS/MS approach is also provided. (iv) homogenic synthetic sphingolipids and their analogs for use in cellular, in vitro and in vivo studies. Currently, core provides stereoisomers of modified sphingoid bases, ceramides and their phosphates, sphingomyelin, modulators of sphingolipid metabolism and advanced molecular probes: functionalized, fluorescent, side-specific radioactive sphingolipids and 17C-backbone sphingolipids. Additionally, analytical approach to study sphingolipid metabolism and enzymatic activities (using sphingolipids composed of 17C-backbone and MS analysis) is also available for the COBRE investigators. Starting from July, 2010 up to April 06, 2011, the analytical subCore performed 2612 analyses for the current COBRE projects (analytical limit established for each project was estimated as 300 analyses/year for each project) and 477 analyses for the former COBRE graduates: Dr. Argraves (87), Dr. Hammad (66), Dr. Hama (44) and Dr. Gudz (280).