EX VIVO MODEL TO STUDY NANOPARTICLE UPTAKE FOR ORAL GENE DELIVERY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目旨在确定在闭合肠环模型中口服后，用量子点标记的聚合物纳米络合物的生物分布。作为一项试点研究，在远红区发射的量子点将被用来标记DNA，然后用DNA与壳聚糖形成纳米络合物。然后，将纳米颗粒注射到大鼠胃肠道(GIT)的分离部分，持续预定的时间。在切除了GIT的相关部分之后，我们建议通过柯达体内成像系统来确定纳米复合体的摄取。该系统将提供GIT中显示增强吸收我们的纳米复合体的特定部分的半定量鉴定。 这项研究将使我们能够更好地设计聚合基因载体和大分子制剂，进一步保护DNA和纳米复合体，以促进更有效的口服基因输送。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The project aims to determine the biodistribution of polymeric nanocomplexes labeled with quantum dots, following oral administration in a closed intestinal loop model. As a pilot study, quantum dots that emit in the far-red region will be used to label DNA, which is then used to form nanocomplexes with chitosan. The nanoparticles are then administered to isolated sections of rat gastrointestinal tract (GIT) for pre-determined durations. Following the excision of the relevant sections of the GIT, we propose to determine the uptake of the nanocomplexes by means of the Kodak In-Vivo Imaging System. The system would provide a semi-quantitative identification of the specific sections of the GIT that show enhanced uptake of our nanocomplexes. Such a study would enable us to better design polymeric gene carriers and macroformulations that would further protect the DNA and nanocomplexesin order to facilitate more efficient oral gene delivery.