IN VIVO CHARACTERIZATION OF RNA VIRUS ASSEMBLY LINES WITH EM TOMOGRAPHY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 病毒是世界上许多人类疾病的原因，并给社会造成重大经济损失。在美国，流感这种人类病原体每年造成的直接和间接损失估计达100亿美元，而未来一场严重的大流行病的成本可能高达数千亿美元。研究病毒组装和进入将有助于研究人员开发新的抗病毒靶点和疫苗。该提案的重点是使用电子显微镜断层扫描来回答有关RNA病毒在体内生命周期的关键问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Viruses are the cause of many human diseases through out the world, and result in major financial losses to society. In the United States Influenza, a human pathogen, is responsible for an estimated total direct and indirect cost of $10 billion per year, and the costs of a serious future pandemic could cost hundreds of billions of dollars. Investigating virus assembly and entry will help researchers develop novel anti-viral targets and vaccines. The focus of this proposal is to use to electron microscope tomography to answer key questions about RNA virus lifecycle in vivo.