SWEET-RECEPTOR SATURATION TRANSFER DIFFERENCE TITRATION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 其具体目的是：1.甜蛋白-受体相互作用的体外细胞分析。2.利用核磁共振波谱研究辣素与甜味感受器相互作用和激活的构象和动态要求。这些研究将有助于确定与功能特性相关的变化。3.用STDD-核磁共振法检测甜蛋白T1R2/T1R3甜味受体及其突变体与Brazzein及其突变体的结合。这些结果将有助于准确地确定Brazzein-Sweet受体相互作用的基本分子特征以及由此产生的信号转导非热量甜味剂，以此作为帮助解决糖尿病和相关疾病的方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The specific aims are: 1. In vitro cellular assays of sweet protein-receptor interactions. 2. To use NMR spectroscopy to investigate the conformational and dynamic requirements in brazzein for its interaction and activation of the sweet taste receptor. These studies will serve to identify changes that correlate with fuctional properties. 3. To monitor binding of brazzein and its mutants to T1R2/T1R3 sweet receptor and its mutants by STDD-NMR spectroscopy. These results will contribute to accurately define the essential molecular features responsible for the brazzein-sweet receptor interaction and the resulting signal transduction non-caloric sweeteners as an approach to help addressing diabetes and related disorders.