NMR OF 6 SAMPLES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 方法： 核磁共振 样品(20 Mg)从99.9%D2O中冷冻干燥换氢，溶于0.7mLD2O(99.96%D)中，加入100°L 10 mg/mLDSS(99.96%D)，放入5 mm核磁共振管中。一维质子核磁共振谱是在瓦里安Inova-500 MHz仪器上在40°C下获得的，谱宽为4.7 kHz，发射机频率设置为-587 Hz，每次实验收集32次扫描。数据用Mestre-C软件进行指数乘法(0.2 Hz)处理。光谱参照最上场的DSS信号(0.0ppm)。用三次Bernstein多项式对光谱进行基线校正。通过积分5.97ppm(UA2S-H4)和5.79ppm(UA-H4)的信号来确定UA2S-H4与UA-H4的比率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Methods: NMR The samples (20 mg) were deuterium-exchanged by lyophilization from D2O (99.9 % D), dissolved in 0.7 mL D2O (99.96 % D), 100 ¿L 10 mg/mL DSS in D2O (99.96 % D) was added, and the solution placed into a 5-mm NMR tube. One-dimensional proton NMR spectra were acquired on a Varian Inova-500 MHz instrument at 40 ¿C. Spectral width was 4.7 kHz, transmitter frequency was set at -587 Hz, and 32 scans were collected in each experiment. The data were processed using the Mestre-C software using exponential multiplication (0.2 Hz). Spectra were referenced to the most upfield DSS signal (0.0 ppm). The spectra were baseline corrected with a third order Bernstein polynomial. The ratio of ¿UA2S-H4 to ¿UA-H4 was determined by integration of the signals at 5.97 ppm ( ¿UA2S-H4) and 5.79 ppm (¿UA-H4).