O-GLCNAC IN EMBRYONIC STEM CELLS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 已知Sox 2以及许多其他转录因子是O-GlcNAc酰化的。 为了更好地理解多能性维持期间的O-GlcNAc化，必须能够i）无偏倚地鉴定所涉及的低丰度O-GlcNAc化核蛋白，ii）为O-GlcNAc化分配位点特异性残基，以及iii）理解O-GlcNAc信号来自什么细胞背景，因为许多核事件来自核内信号。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Sox2, as well as many other transcription factors, are known to be O-GlcNAcylated. In order to better understand O-GlcNAcylation during pluripotency maintenance, one must be able to i) identify the low abudnace O-GlcNAcylated nuclear proteins involved without bias, ii) assign site-specific residues for O-GlcNAcylation and iii) understand from what cellular context the O-GlcNAc signal came, since many nuclear events come from extranuclear signals.