HIGH ENERGY DIFFRACTION DEVELOPMENT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 小角X射线光纤衍射是在获取肌肉生理信息的同时获取结构信息的一种选择方法。进展的一个重要障碍是肌肉对18ID光束线典型的高X射线剂量率下的辐射损伤的敏感性。减轻辐射损害的主要策略是使用相对较大的样本，并在曝光期间将其扫描到光束中。对于小样本来说，这是不可能的，比如那些从啮齿动物心脏中分离出来的样本，所以显然需要一些其他方法来减少损害。来自日本Spring-8研究人员的轶事信息表明，活着的肌肉在15kev时比在12kev时持续时间长得多，但这一点在同行评议的文献中没有被记录下来。在这里，我们探索了在15keV下使用18ID进行肌肉实验的可能性，并查看这是否对样本寿命有显著影响

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Small-angle x-ray fiber diffraction is the method of choice to obtain structural information at the same time as physiological information from working muscle. A significant barrier to progress is the susceptibility of muscle to radiation damage at the high x-ray dose rates typical at beamline 18ID. The main strategy available to mitigate radiation damage is to use a relatively large sample and scan it in the beam during the exposure. This isn't possible with small samples such as those isolated form the hearts of rodents so some other way of reducing damage is clearly needed. Anecdotal information from researchers at Spring-8 in Japan have indicated that living muscles last much longer at 15 keV than at 12 keV but this has not been documented in the peer reviewed literature. Here we explored the possibility of using 18 ID to do muscle experiments at 15 keV and to see if this had appreciable effects on sample lifetimes